Elevated sperm DNA fragmentation does not predict recurrent implantation failure

Jordan C. Best, Taylor Kohn, Premal Patel, Ruben Blachman-Braun, Elaine de Quadros, Zeki Beyhan, Michael Jacobs, Ranjith Ramasamy

Research output: Contribution to journalArticlepeer-review


In this study, we sought to determine whether sperm DNA fragmentation (DFI%) and high DNA stainability (HDS%) evaluated by sperm chromatin structure assay (SCSA) predict recurrent implantation failure (RIF) or pregnancy rate. A retrospective study was performed of consecutive cycles of ICSI treatment from 2009 to 2018. A total of 386 couples that underwent 1,216 frozen embryo transfer (FET) cycles were analysed. Mean female and male age was 34 ± 3.6 years and 37.3 ± 6.6 years, respectively, and a median total motile sperm count (TMSC) was 43.5 [9.9–105.5] million. Overall median DFI% and HDS% was 12 [7.1–18.9] and 9.6 [6.5–14.4] respectively. On multivariable analysis, DFI% and HDS% were not associated with RIF (DFI%: OR = 1.01, 95% CI: 0.98–1.04, p =.414; HDS%: OR = 0.97, 95% CI: 0.94–1.01, p =.107) or IVF success, defined as clinical pregnancy (DFI%: OR = 1.00, 95% CI: 0.99–1.01, p =.641; HDS%: OR = 1.01, 95% CI: 0.99–1.02, p =.565). We found that neither DFI% or HDS%, as assessed by SCSA, were predictive of RIF or pregnancy rate. This finding suggests that sperm DNA fragmentation does not predict RIF or pregnancy rate.

Original languageEnglish (US)
Article numbere14094
Issue number7
StatePublished - Aug 2021


  • DNA fragmentation
  • high DNA stainability
  • in vitro fertilization
  • intracytoplasmic sperm injection
  • recurrent implantation failure

ASJC Scopus subject areas

  • Endocrinology
  • Urology


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