Elevated levels of RhoA in the optic nerve head of human eyes with glaucoma

Brian Goldhagen, Alan D. Proia, David L. Epstein, Ponugoti Vasantha Rao

Research output: Contribution to journalReview article

31 Scopus citations

Abstract

PURPOSE: To determine changes in the expression profile of RhoA and Rho kinase (ROCK-1 and ROCK-2) in the aqueous humor outflow pathway and optic nerve head (ONH) of human eyes with or without glaucoma to explore their potential involvement in glaucoma pathophysiology. METHODS: Age-matched paraffin-embedded postmortem eyes from patients with or without glaucoma were stained immunohistochemically using polyclonal antibodies raised against RhoA, ROCK-1, and ROCK-2. The intensity of the immunostaining in the aqueous humor outflow pathway and the ONH was graded by 4 individuals who were masked concerning whether the eyes were from normal individuals or those with glaucoma. RESULTS: Both normal eyes and those with glaucoma showed a positive staining for RhoA, ROCK-1, and ROCK-2 in the trabecular meshwork, ciliary muscle, and ONH. There was a significant increase in the RhoA protein levels in the glaucomatous ONH compared with the age-matched controls. CONCLUSIONS: Elevated levels of RhoA in the ONH of glaucomatous eyes suggest possible involvement of RhoA in the pathophysiology of glaucoma.

Original languageEnglish (US)
Pages (from-to)530-538
Number of pages9
JournalJournal of glaucoma
Volume21
Issue number8
DOIs
StatePublished - Oct 1 2012
Externally publishedYes

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Keywords

  • Rho GTPase
  • Rho kinase
  • glaucoma
  • optic nerve head

ASJC Scopus subject areas

  • Ophthalmology

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