Elevated levels of RhoA in the optic nerve head of human eyes with glaucoma

Brian Goldhagen, Alan D. Proia, David L. Epstein, Ponugoti Vasantha Rao

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

PURPOSE: To determine changes in the expression profile of RhoA and Rho kinase (ROCK-1 and ROCK-2) in the aqueous humor outflow pathway and optic nerve head (ONH) of human eyes with or without glaucoma to explore their potential involvement in glaucoma pathophysiology. METHODS: Age-matched paraffin-embedded postmortem eyes from patients with or without glaucoma were stained immunohistochemically using polyclonal antibodies raised against RhoA, ROCK-1, and ROCK-2. The intensity of the immunostaining in the aqueous humor outflow pathway and the ONH was graded by 4 individuals who were masked concerning whether the eyes were from normal individuals or those with glaucoma. RESULTS: Both normal eyes and those with glaucoma showed a positive staining for RhoA, ROCK-1, and ROCK-2 in the trabecular meshwork, ciliary muscle, and ONH. There was a significant increase in the RhoA protein levels in the glaucomatous ONH compared with the age-matched controls. CONCLUSIONS: Elevated levels of RhoA in the ONH of glaucomatous eyes suggest possible involvement of RhoA in the pathophysiology of glaucoma.

Original languageEnglish (US)
Pages (from-to)530-538
Number of pages9
JournalJournal of Glaucoma
Volume21
Issue number8
DOIs
StatePublished - Oct 2012
Externally publishedYes

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Optic Disk
Glaucoma
Aqueous Humor
rhoA GTP-Binding Protein
Trabecular Meshwork
rho-Associated Kinases
Paraffin
Staining and Labeling
Muscles
Antibodies

Keywords

  • glaucoma
  • optic nerve head
  • Rho GTPase
  • Rho kinase

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Elevated levels of RhoA in the optic nerve head of human eyes with glaucoma. / Goldhagen, Brian; Proia, Alan D.; Epstein, David L.; Rao, Ponugoti Vasantha.

In: Journal of Glaucoma, Vol. 21, No. 8, 10.2012, p. 530-538.

Research output: Contribution to journalArticle

Goldhagen, Brian ; Proia, Alan D. ; Epstein, David L. ; Rao, Ponugoti Vasantha. / Elevated levels of RhoA in the optic nerve head of human eyes with glaucoma. In: Journal of Glaucoma. 2012 ; Vol. 21, No. 8. pp. 530-538.
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AB - PURPOSE: To determine changes in the expression profile of RhoA and Rho kinase (ROCK-1 and ROCK-2) in the aqueous humor outflow pathway and optic nerve head (ONH) of human eyes with or without glaucoma to explore their potential involvement in glaucoma pathophysiology. METHODS: Age-matched paraffin-embedded postmortem eyes from patients with or without glaucoma were stained immunohistochemically using polyclonal antibodies raised against RhoA, ROCK-1, and ROCK-2. The intensity of the immunostaining in the aqueous humor outflow pathway and the ONH was graded by 4 individuals who were masked concerning whether the eyes were from normal individuals or those with glaucoma. RESULTS: Both normal eyes and those with glaucoma showed a positive staining for RhoA, ROCK-1, and ROCK-2 in the trabecular meshwork, ciliary muscle, and ONH. There was a significant increase in the RhoA protein levels in the glaucomatous ONH compared with the age-matched controls. CONCLUSIONS: Elevated levels of RhoA in the ONH of glaucomatous eyes suggest possible involvement of RhoA in the pathophysiology of glaucoma.

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