Abstract
PURPOSE: To determine changes in the expression profile of RhoA and Rho kinase (ROCK-1 and ROCK-2) in the aqueous humor outflow pathway and optic nerve head (ONH) of human eyes with or without glaucoma to explore their potential involvement in glaucoma pathophysiology. METHODS: Age-matched paraffin-embedded postmortem eyes from patients with or without glaucoma were stained immunohistochemically using polyclonal antibodies raised against RhoA, ROCK-1, and ROCK-2. The intensity of the immunostaining in the aqueous humor outflow pathway and the ONH was graded by 4 individuals who were masked concerning whether the eyes were from normal individuals or those with glaucoma. RESULTS: Both normal eyes and those with glaucoma showed a positive staining for RhoA, ROCK-1, and ROCK-2 in the trabecular meshwork, ciliary muscle, and ONH. There was a significant increase in the RhoA protein levels in the glaucomatous ONH compared with the age-matched controls. CONCLUSIONS: Elevated levels of RhoA in the ONH of glaucomatous eyes suggest possible involvement of RhoA in the pathophysiology of glaucoma.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 530-538 |
| Number of pages | 9 |
| Journal | Journal of Glaucoma |
| Volume | 21 |
| Issue number | 8 |
| DOIs | |
| State | Published - Oct 2012 |
| Externally published | Yes |
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Keywords
- glaucoma
- optic nerve head
- Rho GTPase
- Rho kinase
ASJC Scopus subject areas
- Ophthalmology
Cite this
Elevated levels of RhoA in the optic nerve head of human eyes with glaucoma. / Goldhagen, Brian; Proia, Alan D.; Epstein, David L.; Rao, Ponugoti Vasantha.
In: Journal of Glaucoma, Vol. 21, No. 8, 10.2012, p. 530-538.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Elevated levels of RhoA in the optic nerve head of human eyes with glaucoma
AU - Goldhagen, Brian
AU - Proia, Alan D.
AU - Epstein, David L.
AU - Rao, Ponugoti Vasantha
PY - 2012/10
Y1 - 2012/10
N2 - PURPOSE: To determine changes in the expression profile of RhoA and Rho kinase (ROCK-1 and ROCK-2) in the aqueous humor outflow pathway and optic nerve head (ONH) of human eyes with or without glaucoma to explore their potential involvement in glaucoma pathophysiology. METHODS: Age-matched paraffin-embedded postmortem eyes from patients with or without glaucoma were stained immunohistochemically using polyclonal antibodies raised against RhoA, ROCK-1, and ROCK-2. The intensity of the immunostaining in the aqueous humor outflow pathway and the ONH was graded by 4 individuals who were masked concerning whether the eyes were from normal individuals or those with glaucoma. RESULTS: Both normal eyes and those with glaucoma showed a positive staining for RhoA, ROCK-1, and ROCK-2 in the trabecular meshwork, ciliary muscle, and ONH. There was a significant increase in the RhoA protein levels in the glaucomatous ONH compared with the age-matched controls. CONCLUSIONS: Elevated levels of RhoA in the ONH of glaucomatous eyes suggest possible involvement of RhoA in the pathophysiology of glaucoma.
AB - PURPOSE: To determine changes in the expression profile of RhoA and Rho kinase (ROCK-1 and ROCK-2) in the aqueous humor outflow pathway and optic nerve head (ONH) of human eyes with or without glaucoma to explore their potential involvement in glaucoma pathophysiology. METHODS: Age-matched paraffin-embedded postmortem eyes from patients with or without glaucoma were stained immunohistochemically using polyclonal antibodies raised against RhoA, ROCK-1, and ROCK-2. The intensity of the immunostaining in the aqueous humor outflow pathway and the ONH was graded by 4 individuals who were masked concerning whether the eyes were from normal individuals or those with glaucoma. RESULTS: Both normal eyes and those with glaucoma showed a positive staining for RhoA, ROCK-1, and ROCK-2 in the trabecular meshwork, ciliary muscle, and ONH. There was a significant increase in the RhoA protein levels in the glaucomatous ONH compared with the age-matched controls. CONCLUSIONS: Elevated levels of RhoA in the ONH of glaucomatous eyes suggest possible involvement of RhoA in the pathophysiology of glaucoma.
KW - glaucoma
KW - optic nerve head
KW - Rho GTPase
KW - Rho kinase
UR - http://www.scopus.com/inward/record.url?scp=84867229440&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84867229440&partnerID=8YFLogxK
U2 - 10.1097/IJG.0b013e318241b83c
DO - 10.1097/IJG.0b013e318241b83c
M3 - Article
VL - 21
SP - 530
EP - 538
JO - Journal of Glaucoma
JF - Journal of Glaucoma
SN - 1057-0829
IS - 8
ER -