Abstract
The electroretinographic responses of regenerated eyes of snails (C. aspersa) were studied by means of suction electrodes and single or repetitive flash stimulation. The eyes were fixed and observed under light and electron microscopy. The results indicate that the electroretinographic response of regenerated eyes does not differ from control eyes after dark adaptation. However, the repetitive stimulation of the regenerated eyes induced an earlier fatigue of the response, evident after the fifth stimulus. This fatigue is a function of light intensity. Ultrastructural features of the regenerated eyes are similar to those of the control eyes but regenerated eyes show smaller rhabdomeres, fewer photic vesicles, and fewer paracrystalline bodies. It is suggested that the regenerated eye lability to the repetitive stimulation might be due to the decrease in the amount of photic vesicles and paracrystalline bodies, to the decrease in membrane surface of the rhabdomeres, or to some other metabolic failure not distinguished at electron microscopic level, like the membrane ionic regulation. The appearance of photic vesicles in the axon cones of regenerated and stimulated cells also suggests a possible failure in the mechanism of transport of vesicles.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 167-176 |
| Number of pages | 10 |
| Journal | Journal of Neurobiology |
| Volume | 14 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1983 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Neuroscience(all)
- Cellular and Molecular Neuroscience
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Electroretinographic and ultrastructural study of the regenerated eye of the snail cryptomphallus aspersa. / Flores, D. Vladimir; Salas, Pedro J; Vedra, Jorge Pecci Saa.
In: Journal of Neurobiology, Vol. 14, No. 3, 1983, p. 167-176.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Electroretinographic and ultrastructural study of the regenerated eye of the snail cryptomphallus aspersa
AU - Flores, D. Vladimir
AU - Salas, Pedro J
AU - Vedra, Jorge Pecci Saa
PY - 1983
Y1 - 1983
N2 - The electroretinographic responses of regenerated eyes of snails (C. aspersa) were studied by means of suction electrodes and single or repetitive flash stimulation. The eyes were fixed and observed under light and electron microscopy. The results indicate that the electroretinographic response of regenerated eyes does not differ from control eyes after dark adaptation. However, the repetitive stimulation of the regenerated eyes induced an earlier fatigue of the response, evident after the fifth stimulus. This fatigue is a function of light intensity. Ultrastructural features of the regenerated eyes are similar to those of the control eyes but regenerated eyes show smaller rhabdomeres, fewer photic vesicles, and fewer paracrystalline bodies. It is suggested that the regenerated eye lability to the repetitive stimulation might be due to the decrease in the amount of photic vesicles and paracrystalline bodies, to the decrease in membrane surface of the rhabdomeres, or to some other metabolic failure not distinguished at electron microscopic level, like the membrane ionic regulation. The appearance of photic vesicles in the axon cones of regenerated and stimulated cells also suggests a possible failure in the mechanism of transport of vesicles.
AB - The electroretinographic responses of regenerated eyes of snails (C. aspersa) were studied by means of suction electrodes and single or repetitive flash stimulation. The eyes were fixed and observed under light and electron microscopy. The results indicate that the electroretinographic response of regenerated eyes does not differ from control eyes after dark adaptation. However, the repetitive stimulation of the regenerated eyes induced an earlier fatigue of the response, evident after the fifth stimulus. This fatigue is a function of light intensity. Ultrastructural features of the regenerated eyes are similar to those of the control eyes but regenerated eyes show smaller rhabdomeres, fewer photic vesicles, and fewer paracrystalline bodies. It is suggested that the regenerated eye lability to the repetitive stimulation might be due to the decrease in the amount of photic vesicles and paracrystalline bodies, to the decrease in membrane surface of the rhabdomeres, or to some other metabolic failure not distinguished at electron microscopic level, like the membrane ionic regulation. The appearance of photic vesicles in the axon cones of regenerated and stimulated cells also suggests a possible failure in the mechanism of transport of vesicles.
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U2 - 10.1002/neu.480140302
DO - 10.1002/neu.480140302
M3 - Article
C2 - 6854314
AN - SCOPUS:0020756632
VL - 14
SP - 167
EP - 176
JO - Developmental Neurobiology
JF - Developmental Neurobiology
SN - 0022-3034
IS - 3
ER -