Electrophoretic mobility studies on doxorubicin‐resistant and ‐sensitive murine P338 leukemic cells

Swagata Nair, Allan Horton, Robert C. Leif, Awtar Krishan

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The electrokinetic properties of doxorubicin (DOX)‐resistant (P388/R) and ‐sensitive (P388/S) murine leukemic cells were studied in a free‐flow electrophoresis (FFE) system. The electrophoretic mobilities (EM) of P388/S and P388/R cells were 1.07 and 1.35 × 10−4 cm2 V−1 S−1, respectively, suggesting a higher net negative charge on the P388/R cells. Neuraminidase treatment decreased the EM of both the P388/S and P388/R cells by 15–20% but had no effect on cellular doxorubicin retention. Total and cell surface sialic acid contents were similar in both the cell lines. Our studies show that no direct correlations may exist among surface charge, cell surface sialic acid content, and doxorubicin retention in DOX‐resistant and ‐sensitive P388 cells; however, differences in cell surface charge between these cell types were used to separate them by preparative FFE.

Original languageEnglish (US)
Pages (from-to)232-237
Number of pages6
JournalCytometry
Volume9
Issue number3
DOIs
StatePublished - May 1988

Keywords

  • Flow cytometry
  • cell sorting
  • drug resistance
  • sialic acid
  • tumor cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

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