Electronic volume of CD34 positive cells from peripheral blood apheresis samples

Sherry Shariatmadar, Siddharth Sharma, Raquel Cabana, Scott Powell, Phillip Ruiz, Awtar Krishan

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Background: The authors have used a flow analyzer to measure electronic cellular volume of peripheral blood hematopoietic stem/progenitor cells obtained by granulocyte-colony stimulating factor (G-CSF) mobilization and apheresis (HPC-A) of patients with hematological malignancies. Methods: Fifty three apheresis samples stained with CD45-fluorescein isothiocyanate (FITC) and CD34-R-phycoerythrin (PE)-labeled antibodies after erythrocyte lysis with BD FACS™ Lysing Solution were analyzed for electronic cell volume and two-color FITC and PE fluorescence. Results: Lymphocytes, monocytes and granulocytes in the HPC-A samples had a mean electronic volume of 414, 797, and 670 μm3, respectively corresponding to cell diameter of 9.25, 11.5, and 10.85 μm. In 53 HPC-A samples analyzed, the mean electronic volume of the CD34 positive mononuclear cells was 407 μm3 while the CD45 positive cells had mean volume of 453 μm3. Conclusions: CD34 positive stem/progenitor cells have a smaller volume and diameter than CD45 positive mononuclear cells in HPC-A samples. In the present study the CD34 stem/progenitor cells had a considerably larger diameter than that of stem cells previously reported in the literature. With the availability of electronic cell volume as a parameter in flow cytometric analysis, further studies can be carried out to correlate stem cell volume with specific phenotypic marker expression.

Original languageEnglish (US)
Pages (from-to)182-188
Number of pages7
JournalCytometry Part B - Clinical Cytometry
Volume74
Issue number3
DOIs
StatePublished - May 1 2008

Keywords

  • CD34 antigen
  • Electronic cell volume
  • Hematopoietic progenitors
  • Stem cells

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

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