Electron-transfer properties of cytochrome c Langmuir-Blodgett films and interactions of cytochrome c with lipids

S. Boussaad, L. Dziri, R. Arechabaleta, N. J. Tao, R. M. Leblanc

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

The structural and electron-transfer properties of cytochrome c (Cyt c) Langmuir-Blodgett (LB) films have been studied on graphite electrode with tapping mode atomic force microscopy and cyclic voltammetry (CV). Cyt c in the LB films forms an ordered monolayer in which the individual proteins pack into a quasi-hexagonal structure. The monolayer undergoes a reversible electron-transfer reaction in phosphate buffer. The interactions of Cyt c with cardiolipin (CL) and phosphatidylcholine (PC) LB films have been studied. The LB films of CL and PC are both ordered on graphite, but their interactions with Cyt c are quite different. On a CL monolayer, Cyt c adsorbs spontaneously and the adsorbed protein preserves the electron-transfer reaction. However, on a PC monolayer, Cyt c does not adsorb.

Original languageEnglish (US)
Pages (from-to)6215-6219
Number of pages5
JournalLangmuir
Volume14
Issue number21
StatePublished - Oct 13 1998

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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