Electron paramagnetic resonance (EPR) spectroscopy for investigating murine telogen skin after spontaneous or depilation-induced hair growth

Przemyslaw M. Plonka, Dominika Michalczyk, Malgorzata Popik, Bori Handjiski, Ralf Paus

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Depilation has greatly promoted our understanding of hair follicle biology, however, only marginally of telogen (the "resting" stage of the hair cycle). Since electron paramagnetic resonance (EPR) spectroscopy provides an instructive technique for analyzing hair biology, it may be useful for telogen research. Objectives: To identify differences in murine telogen skin after a spontaneous and depilation-induced hair follicle cycling, and to analyze applicability of EPR to investigate telogen. Methods: Spontaneous or depilation-induced hair cycling in C57BL/6 mice. EPR spectroscopy of unshaven skin and of shaved hair shafts, microscopical examination of plucked or shed hair shafts, standardized histomorphometry. Results: Melanin EPR signals did not differ qualitatively between the two examined types of skin, nor did depilation change the hair length. However, unmanipulated telogen skin revealed greater thickness, stronger EPR signals, 25% more hair shafts, and lower melanin content of individual hair shafts, as creating a much more intricate mosaic of telogen hair follicles with various numbers of hair shafts (0-3) than the skin after depilation-induced hair growth. In both types of skin empty pilary canals were found. Both groups of animals lost hair shafts which were typical of exogen (the actively controlled process of hair shedding). Conclusions: EPR spectroscopy can be profitably employed to study telogen. Murine telogen skin reveals a kenogen-like phenomenon (the "lag" phase following telogen and exogen when hair follicles remain empty, i.e. are devoid of hair shafts). Murine skin thickness in telogen and individual hair shaft pigmentation depend on the way of hair growth induction. Telogens after a spontaneous or depilation-induced hair growth are biologically distinct.

Original languageEnglish (US)
Pages (from-to)227-240
Number of pages14
JournalJournal of Dermatological Science
Volume49
Issue number3
DOIs
StatePublished - Mar 1 2008
Externally publishedYes

Fingerprint

Hair Removal
Electron Spin Resonance Spectroscopy
Hair
Paramagnetic resonance
Spectrum Analysis
Skin
Spectroscopy
Growth
Melanins
Hair Follicle
Canals
Animals

Keywords

  • C57BL/6
  • Exogen
  • Hair cycling
  • Kenogen
  • Melanin
  • Proteases

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Electron paramagnetic resonance (EPR) spectroscopy for investigating murine telogen skin after spontaneous or depilation-induced hair growth. / Plonka, Przemyslaw M.; Michalczyk, Dominika; Popik, Malgorzata; Handjiski, Bori; Paus, Ralf.

In: Journal of Dermatological Science, Vol. 49, No. 3, 01.03.2008, p. 227-240.

Research output: Contribution to journalArticle

Plonka, Przemyslaw M. ; Michalczyk, Dominika ; Popik, Malgorzata ; Handjiski, Bori ; Paus, Ralf. / Electron paramagnetic resonance (EPR) spectroscopy for investigating murine telogen skin after spontaneous or depilation-induced hair growth. In: Journal of Dermatological Science. 2008 ; Vol. 49, No. 3. pp. 227-240.
@article{2a047e74e84e4782b355020b3151da2a,
title = "Electron paramagnetic resonance (EPR) spectroscopy for investigating murine telogen skin after spontaneous or depilation-induced hair growth",
abstract = "Background: Depilation has greatly promoted our understanding of hair follicle biology, however, only marginally of telogen (the {"}resting{"} stage of the hair cycle). Since electron paramagnetic resonance (EPR) spectroscopy provides an instructive technique for analyzing hair biology, it may be useful for telogen research. Objectives: To identify differences in murine telogen skin after a spontaneous and depilation-induced hair follicle cycling, and to analyze applicability of EPR to investigate telogen. Methods: Spontaneous or depilation-induced hair cycling in C57BL/6 mice. EPR spectroscopy of unshaven skin and of shaved hair shafts, microscopical examination of plucked or shed hair shafts, standardized histomorphometry. Results: Melanin EPR signals did not differ qualitatively between the two examined types of skin, nor did depilation change the hair length. However, unmanipulated telogen skin revealed greater thickness, stronger EPR signals, 25{\%} more hair shafts, and lower melanin content of individual hair shafts, as creating a much more intricate mosaic of telogen hair follicles with various numbers of hair shafts (0-3) than the skin after depilation-induced hair growth. In both types of skin empty pilary canals were found. Both groups of animals lost hair shafts which were typical of exogen (the actively controlled process of hair shedding). Conclusions: EPR spectroscopy can be profitably employed to study telogen. Murine telogen skin reveals a kenogen-like phenomenon (the {"}lag{"} phase following telogen and exogen when hair follicles remain empty, i.e. are devoid of hair shafts). Murine skin thickness in telogen and individual hair shaft pigmentation depend on the way of hair growth induction. Telogens after a spontaneous or depilation-induced hair growth are biologically distinct.",
keywords = "C57BL/6, Exogen, Hair cycling, Kenogen, Melanin, Proteases",
author = "Plonka, {Przemyslaw M.} and Dominika Michalczyk and Malgorzata Popik and Bori Handjiski and Ralf Paus",
year = "2008",
month = "3",
day = "1",
doi = "10.1016/j.jdermsci.2007.09.010",
language = "English (US)",
volume = "49",
pages = "227--240",
journal = "Journal of Dermatological Science",
issn = "0923-1811",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Electron paramagnetic resonance (EPR) spectroscopy for investigating murine telogen skin after spontaneous or depilation-induced hair growth

AU - Plonka, Przemyslaw M.

AU - Michalczyk, Dominika

AU - Popik, Malgorzata

AU - Handjiski, Bori

AU - Paus, Ralf

PY - 2008/3/1

Y1 - 2008/3/1

N2 - Background: Depilation has greatly promoted our understanding of hair follicle biology, however, only marginally of telogen (the "resting" stage of the hair cycle). Since electron paramagnetic resonance (EPR) spectroscopy provides an instructive technique for analyzing hair biology, it may be useful for telogen research. Objectives: To identify differences in murine telogen skin after a spontaneous and depilation-induced hair follicle cycling, and to analyze applicability of EPR to investigate telogen. Methods: Spontaneous or depilation-induced hair cycling in C57BL/6 mice. EPR spectroscopy of unshaven skin and of shaved hair shafts, microscopical examination of plucked or shed hair shafts, standardized histomorphometry. Results: Melanin EPR signals did not differ qualitatively between the two examined types of skin, nor did depilation change the hair length. However, unmanipulated telogen skin revealed greater thickness, stronger EPR signals, 25% more hair shafts, and lower melanin content of individual hair shafts, as creating a much more intricate mosaic of telogen hair follicles with various numbers of hair shafts (0-3) than the skin after depilation-induced hair growth. In both types of skin empty pilary canals were found. Both groups of animals lost hair shafts which were typical of exogen (the actively controlled process of hair shedding). Conclusions: EPR spectroscopy can be profitably employed to study telogen. Murine telogen skin reveals a kenogen-like phenomenon (the "lag" phase following telogen and exogen when hair follicles remain empty, i.e. are devoid of hair shafts). Murine skin thickness in telogen and individual hair shaft pigmentation depend on the way of hair growth induction. Telogens after a spontaneous or depilation-induced hair growth are biologically distinct.

AB - Background: Depilation has greatly promoted our understanding of hair follicle biology, however, only marginally of telogen (the "resting" stage of the hair cycle). Since electron paramagnetic resonance (EPR) spectroscopy provides an instructive technique for analyzing hair biology, it may be useful for telogen research. Objectives: To identify differences in murine telogen skin after a spontaneous and depilation-induced hair follicle cycling, and to analyze applicability of EPR to investigate telogen. Methods: Spontaneous or depilation-induced hair cycling in C57BL/6 mice. EPR spectroscopy of unshaven skin and of shaved hair shafts, microscopical examination of plucked or shed hair shafts, standardized histomorphometry. Results: Melanin EPR signals did not differ qualitatively between the two examined types of skin, nor did depilation change the hair length. However, unmanipulated telogen skin revealed greater thickness, stronger EPR signals, 25% more hair shafts, and lower melanin content of individual hair shafts, as creating a much more intricate mosaic of telogen hair follicles with various numbers of hair shafts (0-3) than the skin after depilation-induced hair growth. In both types of skin empty pilary canals were found. Both groups of animals lost hair shafts which were typical of exogen (the actively controlled process of hair shedding). Conclusions: EPR spectroscopy can be profitably employed to study telogen. Murine telogen skin reveals a kenogen-like phenomenon (the "lag" phase following telogen and exogen when hair follicles remain empty, i.e. are devoid of hair shafts). Murine skin thickness in telogen and individual hair shaft pigmentation depend on the way of hair growth induction. Telogens after a spontaneous or depilation-induced hair growth are biologically distinct.

KW - C57BL/6

KW - Exogen

KW - Hair cycling

KW - Kenogen

KW - Melanin

KW - Proteases

UR - http://www.scopus.com/inward/record.url?scp=38749145180&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38749145180&partnerID=8YFLogxK

U2 - 10.1016/j.jdermsci.2007.09.010

DO - 10.1016/j.jdermsci.2007.09.010

M3 - Article

VL - 49

SP - 227

EP - 240

JO - Journal of Dermatological Science

JF - Journal of Dermatological Science

SN - 0923-1811

IS - 3

ER -