TY - GEN
T1 - Electrochemical detection of salivary RNA
AU - Wei, Fang
AU - Zimmermann, Bernhard
AU - Li, Na
AU - Ho, Chih Ming
AU - Wong, David T.
PY - 2007/12/1
Y1 - 2007/12/1
N2 - Electrochemical biosensor for salivary RNA detection with high sensitivity is presented. The complexity of saliva and the low concentration of salivary RNA require both high sensitivity and specificity. In this study enzyme amplification is applied to achieve high signal intensity. In order to increase the current signal, effective hybridization and electron transfer are both required. Electrochemical studies on the location of anchor label and report label have been carried out. Results indicate the hybridization efficiency and the surface hindrance to enzyme amplification are the two major factors contributed to the detected current signal. Human salivary IL-8 RNA has been detected. For oligonucleotide target, the limitation of detection (LOD) is about 1 fM, while the LOD for IVT RNA is about 50 pM.
AB - Electrochemical biosensor for salivary RNA detection with high sensitivity is presented. The complexity of saliva and the low concentration of salivary RNA require both high sensitivity and specificity. In this study enzyme amplification is applied to achieve high signal intensity. In order to increase the current signal, effective hybridization and electron transfer are both required. Electrochemical studies on the location of anchor label and report label have been carried out. Results indicate the hybridization efficiency and the surface hindrance to enzyme amplification are the two major factors contributed to the detected current signal. Human salivary IL-8 RNA has been detected. For oligonucleotide target, the limitation of detection (LOD) is about 1 fM, while the LOD for IVT RNA is about 50 pM.
KW - Electrochemical biosensor
KW - Salivary RNA
UR - http://www.scopus.com/inward/record.url?scp=52949131926&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=52949131926&partnerID=8YFLogxK
U2 - 10.1109/NANO.2007.4601151
DO - 10.1109/NANO.2007.4601151
M3 - Conference contribution
AN - SCOPUS:52949131926
SN - 1424406080
SN - 9781424406081
T3 - 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings
SP - 112
EP - 115
BT - 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings
T2 - 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007
Y2 - 2 August 2007 through 5 August 2007
ER -