Electrochemical detection of salivary RNA

Fang Wei, Bernhard Zimmermann, Na Li, Chih Ming Ho, David T. Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Electrochemical biosensor for salivary RNA detection with high sensitivity is presented. The complexity of saliva and the low concentration of salivary RNA require both high sensitivity and specificity. In this study enzyme amplification is applied to achieve high signal intensity. In order to increase the current signal, effective hybridization and electron transfer are both required. Electrochemical studies on the location of anchor label and report label have been carried out. Results indicate the hybridization efficiency and the surface hindrance to enzyme amplification are the two major factors contributed to the detected current signal. Human salivary IL-8 RNA has been detected. For oligonucleotide target, the limitation of detection (LOD) is about 1 fM, while the LOD for IVT RNA is about 50 pM.

Original languageEnglish (US)
Title of host publication2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings
Pages112-115
Number of pages4
DOIs
StatePublished - Dec 1 2007
Event2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007 - Hong Kong, China
Duration: Aug 2 2007Aug 5 2007

Publication series

Name2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings

Other

Other2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007
CountryChina
CityHong Kong
Period8/2/078/5/07

Keywords

  • Electrochemical biosensor
  • Salivary RNA

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics

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  • Cite this

    Wei, F., Zimmermann, B., Li, N., Ho, C. M., & Wong, D. T. (2007). Electrochemical detection of salivary RNA. In 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings (pp. 112-115). [4601151] (2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings). https://doi.org/10.1109/NANO.2007.4601151