Electrical stimulation of embryonic neurons for 1 hour improves axon regeneration and the number of reinnervated muscles that function

Yang Liu, Robert M. Grumbles, Christine K Thomas

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Motoneuron death after spinal cord injury or disease results in muscle denervation, atrophy, and paralysis. We have previously transplanted embryonic ventral spinal cord cells into the peripheral nerve to reinnervate denervated muscles and to reduce muscle atrophy, but reinnervation was incomplete. Here, our aim was to determine whether brief electrical stimulation of embryonic neurons in the peripheralnerve changes motoneuron survival, axon regeneration, and muscle reinnervation and function because neural depolarization is crucial for embryonic neuron survival and may promote activity-dependent axon growth. At 1 week after denervation by sciatic nerve section, embryonic day 14 to 15 cells were purified for motoneurons, injected into the tibial nerve of adult Fischer rats, and stimulated immediatelyfor up to 1 hour. More myelinated axons were present in tibial nerves 10 weeks after transplantation when transplants had been stimulated acutely at 1 Hz for 1 hour. More muscles were reinnervated if the stimulation treatment lasted for 1 hour. Reinnervation reduced muscle atrophy, with or without the stimulation treatment. These data suggest that brief stimulation of embryonic neurons promotes axon growth, which has a long-term impact on muscle reinnervation and function. Muscle reinnervation is important because it may enable the use of functional electrical stimulation to restore limb movements.

Original languageEnglish
Pages (from-to)697-707
Number of pages11
JournalJournal of Neuropathology and Experimental Neurology
Volume72
Issue number7
DOIs
StatePublished - Jul 1 2013

Fingerprint

Electric Stimulation
Axons
Regeneration
Muscular Atrophy
Neurons
Motor Neurons
Muscles
Tibial Nerve
Muscle Denervation
Spinal Cord Diseases
Inbred F344 Rats
Denervation
Sciatic Nerve
Growth
Spinal Cord Injuries
Peripheral Nerves
Paralysis
Spinal Cord
Extremities
Transplantation

Keywords

  • Axon growth cone initiation
  • Cell transplantation
  • Motoneuron survival
  • Muscle denervation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Electrical stimulation of embryonic neurons for 1 hour improves axon regeneration and the number of reinnervated muscles that function. / Liu, Yang; Grumbles, Robert M.; Thomas, Christine K.

In: Journal of Neuropathology and Experimental Neurology, Vol. 72, No. 7, 01.07.2013, p. 697-707.

Research output: Contribution to journalArticle

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