ELECTRICAL and EMISSION PROPERTIES OF CHLOROPHYLL a IN PLANAR ASYMMETRICAL MEMBRANES

Sylvain Robert, Pierre Tancrède, André Houle, Roger Leblanc

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Abstract— We have designed and constructed an experimental set‐up allowing the simultaneous determination of the electrical and fluorescence properties of pigments incorporated in asymmetrical lipid bilayers formed from monolayers. The system is composed of two Langmuir troughs machined in an annealed Teflon block. Each trough is mounted with a movable barrier for the compression of the monolayers as well as with a float type Langmuir torsion balance to measure the surface properties of the monolayers prior to membrane formation. The trough is also equipped with optical windows which permit the localization of a laser beam on the reconstituted membrane incorporating the chromophore. The reconstituted bilayers are further characterized by measurement of their specific resistance and capacitance properties. A detailed description of the experimental method proposed is given, stressing the main advantages of our technique. Finally preliminary results on asymmetrical phosphatidylethanolamine/phosphatidylserine bilayers incorporating chlorophyll‐a molecules are presented and discussed.

Original languageEnglish (US)
Pages (from-to)101-106
Number of pages6
JournalPhotochemistry and Photobiology
Volume41
Issue number1
DOIs
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry
  • Physical and Theoretical Chemistry

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