Efficient internalization of IL-2 depends on the distal portion of the cytoplasmic tail of the IL-2R common γ-chain and a lymphoid cell environment

A. Yu, F. Olosz, C. Y. Choi, Thomas Malek

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27 Citations (Scopus)

Abstract

The common γ-chain (γc), a subunit of the IL-2R, is essential for high affinity ligand binding and signal transduction due to Jak3 association to γc. Another consequence of IL-2/IL-2R interaction is rapid receptor-mediated endocytosis of the receptor-ligand complex. In the present study, we establish that this rapid endocytosis of IL-2 in a T cell tumor line is dependent upon the cytoplasmic tail of γc. Deletion mutants of the cytoplasmic tail mapped this activity to 9 aa of γc, 45-54 aa distal to the transmembrane region. In contrast, ligand-independent constitutive endocytosis of γc occurred more slowly and was dependent upon a PEST sequence in a more membrane-proximal region of the cytoplasmic tail of γc. Thus, this receptor subunit may use distinct sorting signals for its constitutive regulation and ligand-induced endocytosis. Rapid endocytosis of IL-2 was inhibited by the tyrosine kinase inhibitor genistein, implicating a role for a signal transduction pathway in IL-2 internalization. However, one T cell line bearing a mutant γc exhibited impaired endocytosis of IL-2, despite normal IL-2-induced Jak/STAT activation. Furthermore, inefficient endocytosis of IL-2 was noted after transfection of the COS7 epithelial cell line with the IL-2R, and further reconstitution of these cells with Jak/STAT proteins did not enhance this internalization. Collectively, these latter findings indicate that rapid endocytosis of IL-2 is dependent upon cellular signaling in lymphoid cell environment that is not solely a consequence of the presence of the Jak/STAT pathway.

Original languageEnglish
Pages (from-to)2556-2562
Number of pages7
JournalJournal of Immunology
Volume165
Issue number5
StatePublished - Sep 1 2000

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Endocytosis
Interleukin-2
Lymphocytes
Ligands
Cell Line
Signal Transduction
T-Lymphocytes
Genistein
Protein-Tyrosine Kinases
Transfection
Epithelial Cells
Membranes
Neoplasms

ASJC Scopus subject areas

  • Immunology

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Efficient internalization of IL-2 depends on the distal portion of the cytoplasmic tail of the IL-2R common γ-chain and a lymphoid cell environment. / Yu, A.; Olosz, F.; Choi, C. Y.; Malek, Thomas.

In: Journal of Immunology, Vol. 165, No. 5, 01.09.2000, p. 2556-2562.

Research output: Contribution to journalArticle

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