Efficient induction of primary and secondary T cell-dependent immune responses in vivo in the absence of functional IL-2 and IL-15 receptors

Aixin Yu, Jiehao Zhou, Norman Marten, Cornelia C. Bergmann, Michele Mammolenti, Robert B Levy, Thomas Malek

Research output: Contribution to journalArticle

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Abstract

IL-2 and IL-15 are thought to be important cytokines for T cell-dependent immune responses. Mice deficient in IL-2, IL-2Rα, and IL-2Rβ are each characterized by a rapid lethal autoimmune lymphoproliferative disorder that complicates their use in studies aimed at investigating the role of these cytokines and receptors for immune responses in vivo. We have previously characterized a novel transgenic (Tg) mouse on the IL-2Rβ-/- genetic background (Tg-/- mice) that lacks autoimmune disease but still contains peripheral T cells that are nonresponsive to IL-2 and IL-15. In the present study, these mice were used to investigate the extent by which IL-2 and IL-15 are essential for T cell immunity in vivo. Tg-/- mice generated near normal primary and secondary Ab responses to OVA, readily mounted first and second set allogeneic skin graft rejection responses, and developed primary and recall CD8 T cell responses to vaccinia virus. However, Tg-/- mice generated a slightly lower level of IgG2a Abs to OVA, exhibited a somewhat delayed first set skin graft rejection response with lower allo-specific CTL, and developed a significantly lower number of IFN-γ-producing vaccinia-specific CD8+ T cells. Thus, although T effector function is somewhat impaired, T cell immunity is largely functional in the absence of IL-2- and IL-15-induced signaling through IL-2Rβ.

Original languageEnglish
Pages (from-to)236-242
Number of pages7
JournalJournal of Immunology
Volume170
Issue number1
StatePublished - Jan 1 2003

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Interleukin-15 Receptors
Interleukin-2
Interleukin-15
T-Lymphocytes
Transgenic Mice
Graft Rejection
Immunity
Vaccinia
Skin
Cytokine Receptors
Lymphoproliferative Disorders
Vaccinia virus
Autoimmune Diseases
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Efficient induction of primary and secondary T cell-dependent immune responses in vivo in the absence of functional IL-2 and IL-15 receptors. / Yu, Aixin; Zhou, Jiehao; Marten, Norman; Bergmann, Cornelia C.; Mammolenti, Michele; Levy, Robert B; Malek, Thomas.

In: Journal of Immunology, Vol. 170, No. 1, 01.01.2003, p. 236-242.

Research output: Contribution to journalArticle

Yu, Aixin ; Zhou, Jiehao ; Marten, Norman ; Bergmann, Cornelia C. ; Mammolenti, Michele ; Levy, Robert B ; Malek, Thomas. / Efficient induction of primary and secondary T cell-dependent immune responses in vivo in the absence of functional IL-2 and IL-15 receptors. In: Journal of Immunology. 2003 ; Vol. 170, No. 1. pp. 236-242.
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