Efficient in vitro generation of adult multipotent cells from mobilized peripheral blood CD133+ cells

S. Kuçi, Z. Kuçi, S. Schmid, G. Seitz, I. Müller, A. Dufke, T. Leimig, G. Murti, Roland Jurecic, M. Schumm, P. Lang, G. Bruchelt, P. Bader, T. Klingebiel, D. Niethammer, R. Handgretinger

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Abstract

Objectives: To generate non-haematopoietic tissues from mobilized haematopoietic CD133+ stem cells. Materials and methods: Mobilized peripheral blood CD133+ cells from adult healthy donors were used. In vitro ability of highly enriched CD133+ cells from mobilized peripheral blood to generate multipotent cells, and their potential to give rise to cells with characteristics of neuroectoderm, endoderm and mesoderm layers was investigated. Results: We found that a recently identified population of CD45+ adherent cells generated in vitro after culture of highly purified CD133+ cells for 3-5 weeks with Flt3/Flk2 ligand and interleukin-6 can, in presence of the appropriate microenvironmental cues, differentiate into neural progenitor-like cells (NPLCs), hepatocyte-like cells and skeletal muscle-like cells. We have termed them to be adult multipotent haematopoietic cells (AMHCs). AMHC-derived NPLCs expressed morphological, phenotypic and molecular markers associated with primary neural progenitor cells. They can differentiate into astrocyte-like cells, neuronal-like cells and oligodendrocyte-like cells. Moreover, AMHC-derived NPLCs produced 3,4-dihydrophenylalanine and dopamine and expressed voltage-activated ion channels, suggesting their functional maturation. In addition, AMHC-derived hepatocyte-like cells and skeletal muscle-like cells, showed typical morphological features and expressed primary tissue-associated proteins. Conclusion: Our data demonstrate that AMHCs may therefore serve as a novel source of adult multipotent cells for autologous replacement cell therapies.

Original languageEnglish
Pages (from-to)12-27
Number of pages16
JournalCell Proliferation
Volume41
Issue number1
DOIs
StatePublished - Feb 1 2008

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Blood Cells
Stem Cells
In Vitro Techniques
Muscle Cells
Hepatocytes
Skeletal Muscle
Neural Plate
Endoderm
Aptitude
Oligodendroglia
Mesoderm
Cell- and Tissue-Based Therapy
Hematopoietic Stem Cells
Ion Channels
Astrocytes
Cues
Interleukin-6
Dopamine

ASJC Scopus subject areas

  • Cell Biology

Cite this

Kuçi, S., Kuçi, Z., Schmid, S., Seitz, G., Müller, I., Dufke, A., ... Handgretinger, R. (2008). Efficient in vitro generation of adult multipotent cells from mobilized peripheral blood CD133+ cells. Cell Proliferation, 41(1), 12-27. https://doi.org/10.1111/j.1365-2184.2007.00502.x

Efficient in vitro generation of adult multipotent cells from mobilized peripheral blood CD133+ cells. / Kuçi, S.; Kuçi, Z.; Schmid, S.; Seitz, G.; Müller, I.; Dufke, A.; Leimig, T.; Murti, G.; Jurecic, Roland; Schumm, M.; Lang, P.; Bruchelt, G.; Bader, P.; Klingebiel, T.; Niethammer, D.; Handgretinger, R.

In: Cell Proliferation, Vol. 41, No. 1, 01.02.2008, p. 12-27.

Research output: Contribution to journalArticle

Kuçi, S, Kuçi, Z, Schmid, S, Seitz, G, Müller, I, Dufke, A, Leimig, T, Murti, G, Jurecic, R, Schumm, M, Lang, P, Bruchelt, G, Bader, P, Klingebiel, T, Niethammer, D & Handgretinger, R 2008, 'Efficient in vitro generation of adult multipotent cells from mobilized peripheral blood CD133+ cells', Cell Proliferation, vol. 41, no. 1, pp. 12-27. https://doi.org/10.1111/j.1365-2184.2007.00502.x
Kuçi, S. ; Kuçi, Z. ; Schmid, S. ; Seitz, G. ; Müller, I. ; Dufke, A. ; Leimig, T. ; Murti, G. ; Jurecic, Roland ; Schumm, M. ; Lang, P. ; Bruchelt, G. ; Bader, P. ; Klingebiel, T. ; Niethammer, D. ; Handgretinger, R. / Efficient in vitro generation of adult multipotent cells from mobilized peripheral blood CD133+ cells. In: Cell Proliferation. 2008 ; Vol. 41, No. 1. pp. 12-27.
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