Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination

N. F. Tsinoremas, A. K. Kutach, C. A. Strayer, S. S. Golden

Research output: Contribution to journalComment/debatepeer-review

49 Scopus citations

Abstract

We developed a versatile, efficient genetic transfer method for Synechococcus sp. strains PCC 7942 and PCC 6301 that exceeds natural transformation efficiencies by orders of magnitude. As a test case, we complemented a histidine auxotroph and identified a hisS homolog of PCC 7942 as the complementing gene.

Original languageEnglish (US)
Pages (from-to)6764-6768
Number of pages5
JournalJournal of bacteriology
Volume176
Issue number21
DOIs
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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