Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination

N. F. Tsinoremas; A. K. Kutach; C. A. Strayer; S. S. Golden

doi:10.1128/jb.176.21.6764-6768.1994

Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination

N. F. Tsinoremas, A. K. Kutach, C. A. Strayer, S. S. Golden

Research output: Contribution to journal › Comment/debate › peer-review

48 Scopus citations

Abstract

We developed a versatile, efficient genetic transfer method for Synechococcus sp. strains PCC 7942 and PCC 6301 that exceeds natural transformation efficiencies by orders of magnitude. As a test case, we complemented a histidine auxotroph and identified a hisS homolog of PCC 7942 as the complementing gene.

Original language	English (US)
Pages (from-to)	6764-6768
Number of pages	5
Journal	Journal of bacteriology
Volume	176
Issue number	21
DOIs	https://doi.org/10.1128/jb.176.21.6764-6768.1994
State	Published - 1994
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Molecular Biology

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abstract = "We developed a versatile, efficient genetic transfer method for Synechococcus sp. strains PCC 7942 and PCC 6301 that exceeds natural transformation efficiencies by orders of magnitude. As a test case, we complemented a histidine auxotroph and identified a hisS homolog of PCC 7942 as the complementing gene.",

author = "Tsinoremas, {N. F.} and Kutach, {A. K.} and Strayer, {C. A.} and Golden, {S. S.}",

year = "1994",

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AU - Kutach, A. K.

AU - Strayer, C. A.

AU - Golden, S. S.

PY - 1994

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N2 - We developed a versatile, efficient genetic transfer method for Synechococcus sp. strains PCC 7942 and PCC 6301 that exceeds natural transformation efficiencies by orders of magnitude. As a test case, we complemented a histidine auxotroph and identified a hisS homolog of PCC 7942 as the complementing gene.

AB - We developed a versatile, efficient genetic transfer method for Synechococcus sp. strains PCC 7942 and PCC 6301 that exceeds natural transformation efficiencies by orders of magnitude. As a test case, we complemented a histidine auxotroph and identified a hisS homolog of PCC 7942 as the complementing gene.

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DO - 10.1128/jb.176.21.6764-6768.1994

M3 - Comment/debate

C2 - 7961432

AN - SCOPUS:0028081237

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JO - Journal of Bacteriology

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