Efficient c-kit receptor-targeted gene transfer to primary human CD34-selected hematopoietic stem cells

Q. Zhong, P. Oliver, W. Huang, D. Good, V. La Russa, Z. Zhang, J. R. Cork, R. W. Veith, C. Theodossiou, J. K. Kolls, P. Schwarzenberger

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have previously reported effective gene transfer with a targeted molecular conjugate adenovirus vector through the c-kit receptor in hematopoietic progenitor cell lines. However, a c-kit-targeted recombinant retroviral vector failed to transduce cells, indicating the existence of significant differences for c-kit target gene transfer between these two viruses. Here we demonstrate that conjugation of an adenovirus to a c-kit-retargeted retrovirus vector enables retroviral transduction. This finding suggests the requirement of endosomalysis for successful c-kit-targeted gene transfer. Furthermore, we show efficient gene transfer to, and high transgene expression (66%) in, CD34-selected, c-kit+ human peripheral blood stem cells using a c-kit-targeted adenovirus vector. These findings may have important implications for future vector development in c-kit-targeted stem cell gene transfer.

Original languageEnglish
Pages (from-to)10393-10400
Number of pages8
JournalJournal of Virology
Volume75
Issue number21
DOIs
StatePublished - Oct 20 2001

Fingerprint

Proto-Oncogene Proteins c-kit
Hematopoietic Stem Cells
gene transfer
Adenoviridae
receptors
retroviral vectors
Genes
stem cells
Retroviridae
blood cells
Transgenes
transgenes
Stem Cells
cell lines
hematopoietic stem cells
Viruses
Cell Line
viruses
cells

ASJC Scopus subject areas

  • Immunology

Cite this

Zhong, Q., Oliver, P., Huang, W., Good, D., La Russa, V., Zhang, Z., ... Schwarzenberger, P. (2001). Efficient c-kit receptor-targeted gene transfer to primary human CD34-selected hematopoietic stem cells. Journal of Virology, 75(21), 10393-10400. https://doi.org/10.1128/JVI.75.21.10393-10400.2001

Efficient c-kit receptor-targeted gene transfer to primary human CD34-selected hematopoietic stem cells. / Zhong, Q.; Oliver, P.; Huang, W.; Good, D.; La Russa, V.; Zhang, Z.; Cork, J. R.; Veith, R. W.; Theodossiou, C.; Kolls, J. K.; Schwarzenberger, P.

In: Journal of Virology, Vol. 75, No. 21, 20.10.2001, p. 10393-10400.

Research output: Contribution to journalArticle

Zhong, Q, Oliver, P, Huang, W, Good, D, La Russa, V, Zhang, Z, Cork, JR, Veith, RW, Theodossiou, C, Kolls, JK & Schwarzenberger, P 2001, 'Efficient c-kit receptor-targeted gene transfer to primary human CD34-selected hematopoietic stem cells', Journal of Virology, vol. 75, no. 21, pp. 10393-10400. https://doi.org/10.1128/JVI.75.21.10393-10400.2001
Zhong, Q. ; Oliver, P. ; Huang, W. ; Good, D. ; La Russa, V. ; Zhang, Z. ; Cork, J. R. ; Veith, R. W. ; Theodossiou, C. ; Kolls, J. K. ; Schwarzenberger, P. / Efficient c-kit receptor-targeted gene transfer to primary human CD34-selected hematopoietic stem cells. In: Journal of Virology. 2001 ; Vol. 75, No. 21. pp. 10393-10400.
@article{cb294b8f8abf47ee9c991ff055820e0c,
title = "Efficient c-kit receptor-targeted gene transfer to primary human CD34-selected hematopoietic stem cells",
abstract = "We have previously reported effective gene transfer with a targeted molecular conjugate adenovirus vector through the c-kit receptor in hematopoietic progenitor cell lines. However, a c-kit-targeted recombinant retroviral vector failed to transduce cells, indicating the existence of significant differences for c-kit target gene transfer between these two viruses. Here we demonstrate that conjugation of an adenovirus to a c-kit-retargeted retrovirus vector enables retroviral transduction. This finding suggests the requirement of endosomalysis for successful c-kit-targeted gene transfer. Furthermore, we show efficient gene transfer to, and high transgene expression (66{\%}) in, CD34-selected, c-kit+ human peripheral blood stem cells using a c-kit-targeted adenovirus vector. These findings may have important implications for future vector development in c-kit-targeted stem cell gene transfer.",
author = "Q. Zhong and P. Oliver and W. Huang and D. Good and {La Russa}, V. and Z. Zhang and Cork, {J. R.} and Veith, {R. W.} and C. Theodossiou and Kolls, {J. K.} and P. Schwarzenberger",
year = "2001",
month = "10",
day = "20",
doi = "10.1128/JVI.75.21.10393-10400.2001",
language = "English",
volume = "75",
pages = "10393--10400",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "21",

}

TY - JOUR

T1 - Efficient c-kit receptor-targeted gene transfer to primary human CD34-selected hematopoietic stem cells

AU - Zhong, Q.

AU - Oliver, P.

AU - Huang, W.

AU - Good, D.

AU - La Russa, V.

AU - Zhang, Z.

AU - Cork, J. R.

AU - Veith, R. W.

AU - Theodossiou, C.

AU - Kolls, J. K.

AU - Schwarzenberger, P.

PY - 2001/10/20

Y1 - 2001/10/20

N2 - We have previously reported effective gene transfer with a targeted molecular conjugate adenovirus vector through the c-kit receptor in hematopoietic progenitor cell lines. However, a c-kit-targeted recombinant retroviral vector failed to transduce cells, indicating the existence of significant differences for c-kit target gene transfer between these two viruses. Here we demonstrate that conjugation of an adenovirus to a c-kit-retargeted retrovirus vector enables retroviral transduction. This finding suggests the requirement of endosomalysis for successful c-kit-targeted gene transfer. Furthermore, we show efficient gene transfer to, and high transgene expression (66%) in, CD34-selected, c-kit+ human peripheral blood stem cells using a c-kit-targeted adenovirus vector. These findings may have important implications for future vector development in c-kit-targeted stem cell gene transfer.

AB - We have previously reported effective gene transfer with a targeted molecular conjugate adenovirus vector through the c-kit receptor in hematopoietic progenitor cell lines. However, a c-kit-targeted recombinant retroviral vector failed to transduce cells, indicating the existence of significant differences for c-kit target gene transfer between these two viruses. Here we demonstrate that conjugation of an adenovirus to a c-kit-retargeted retrovirus vector enables retroviral transduction. This finding suggests the requirement of endosomalysis for successful c-kit-targeted gene transfer. Furthermore, we show efficient gene transfer to, and high transgene expression (66%) in, CD34-selected, c-kit+ human peripheral blood stem cells using a c-kit-targeted adenovirus vector. These findings may have important implications for future vector development in c-kit-targeted stem cell gene transfer.

UR - http://www.scopus.com/inward/record.url?scp=0034796323&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034796323&partnerID=8YFLogxK

U2 - 10.1128/JVI.75.21.10393-10400.2001

DO - 10.1128/JVI.75.21.10393-10400.2001

M3 - Article

VL - 75

SP - 10393

EP - 10400

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 21

ER -