Efficient c-kit receptor-targeted gene transfer to primary human CD34-selected hematopoietic stem cells

Q. Zhong, P. Oliver, W. Huang, D. Good, V. La Russa, Z. Zhang, J. R. Cork, R. W. Veith, C. Theodossiou, J. K. Kolls, P. Schwarzenberger

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

We have previously reported effective gene transfer with a targeted molecular conjugate adenovirus vector through the c-kit receptor in hematopoietic progenitor cell lines. However, a c-kit-targeted recombinant retroviral vector failed to transduce cells, indicating the existence of significant differences for c-kit target gene transfer between these two viruses. Here we demonstrate that conjugation of an adenovirus to a c-kit-retargeted retrovirus vector enables retroviral transduction. This finding suggests the requirement of endosomalysis for successful c-kit-targeted gene transfer. Furthermore, we show efficient gene transfer to, and high transgene expression (66%) in, CD34-selected, c-kit+ human peripheral blood stem cells using a c-kit-targeted adenovirus vector. These findings may have important implications for future vector development in c-kit-targeted stem cell gene transfer.

Original languageEnglish (US)
Pages (from-to)10393-10400
Number of pages8
JournalJournal of virology
Volume75
Issue number21
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Zhong, Q., Oliver, P., Huang, W., Good, D., La Russa, V., Zhang, Z., Cork, J. R., Veith, R. W., Theodossiou, C., Kolls, J. K., & Schwarzenberger, P. (2001). Efficient c-kit receptor-targeted gene transfer to primary human CD34-selected hematopoietic stem cells. Journal of virology, 75(21), 10393-10400. https://doi.org/10.1128/JVI.75.21.10393-10400.2001