Efficiency of human HLA-mismatched CD34+ cells from unrelated donors in establishing in vitro hematopoiesis in allogeneic long-term marrow cultures

V. F. La Russa, S. W. Kessler, V. A. Henson, M. Cutting, T. Polsinelli, R. D. Knight, D. G. Wright

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We have examined the capacity of highly purified human CD34+ marrow cell isolates from unrelated HLA-mismatched donors to establish in vitro hematopoiesis on recipient marrow stromal cells in 2-stage hematopoietic long-term marrow cultures (H-LTMC). HLA-typing of both peripheral blood mononuclear cells and CD34+ marrow cells was performed for both HLA class I and HLA class II antigens for eight healthy individuals. Significant antigenic mismatches for these molecules ranged from three to six antigens for each recipient-donor pair. Comparison of MHC antigen expression by peripheral blood cells and CD34+ marrow cell isolates confirmed the presence of identical HLA-A, -B, and -C, and -DR specificities on the surface of these cells. Typing of -DQ specificities, however, was not consistently reactive on CD34+ cells. The ≤20% plating efficiency of purified CD34+ cells for BFU-E, CFU-GM, and CPU-MIX allowed us to use inoculum doses of 103, 104, and 105 cells to determine the efficiency of allogeneic CD34+ cells in achieving in vitro engraftment and the establishment of hematopoiesis in H-LTMC. Engraftment of adherent BFU-E, CFU-GM, and CFU-MIX was equally efficient for autologous and allogeneic CD34+ cells. In vitro hematopoiesis reflected by the cumulative recoveries of progenitor cells over time was also equivalent for allogeneic and autologous CD34+ cells. These results demonstrate that highly purified, HLA-mismatched CD34+ marrow cells proliferate and establish in vitro hematopoiesis as efficiently as autologous cells in marrow derived stromal cell cultures and confirm that interactions between stromal cells and highly purified CD34+, DR-, and CD34+, DR+ marrow cell isolates are not MHC-restricted.

Original languageEnglish (US)
Pages (from-to)1475-1483
Number of pages9
JournalExperimental Hematology
Volume24
Issue number13
StatePublished - Dec 23 1996

Keywords

  • CD34 cells
  • MHC restriction
  • Stromal cells

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Fingerprint Dive into the research topics of 'Efficiency of human HLA-mismatched CD34<sup>+</sup> cells from unrelated donors in establishing in vitro hematopoiesis in allogeneic long-term marrow cultures'. Together they form a unique fingerprint.

Cite this