Efficacy of various drugs in the prevention of posterior capsule opacification: Experimental study of rabbit eyes

Viviana Fernandez, Miryam A. Fragoso, Christian Billotte, Peggy Lamar, Marcia A. Orozco, Sander Dubovy, Mark Willcox, Jean-Marie A Parel

Research output: Contribution to journalArticle

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Abstract

To assess the efficacy of various drugs in the prevention of posterior capsule opacification (PCO) in a closed capsular bag technique. Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, Florida, USA. Lens material was removed using phacoaspiration or phacoemulsification through a microcapsulorhexis according to the hardness of the crystalline lens correlated with the weight and age of the rabbits. A mixture of an ophthalmic viscosurgical device (sodium hyaluronate 1.4% [SHA]) and a drug was injected into the empty capsular bag, allowed to remain inside for 3 minutes, and removed. The capsular bag was rinsed with balanced salt solution (BSS®) and refilled with SHA. In a group of rabbits, the capsulorhexis was sealed with a minicapsulorhexis valve (MCV). Rabbits were treated with 1 of the following: SHA (control), BSS, mitomycin-C (MMC, 0.2 mg/mL), ethylenediaminetetraacetic acid (EDTA) (10 mM and 15 mM), 5-fluorouracil (5-FU, 33 mg/mL), acetic acid (3%, 0.3%, and 0.003%), and distilled water. Upon completion of the study, the control and treated eyes had PCO and new lens material (not residual). Anterior capsule proliferation was observed in eyes treated with 5-FU. The order of PCO appearance (earliest to latest) was as follows: 15 mM EDTA, SHA, MMC, acetic acid 0.3%, acetic acid 3%, BSS, distilled water (small animals; no MCV), acetic acid 0.003%, 5-FU, 10 mM EDTA, and distilled water (large animals; MCV). The earliest appearance was day 1 postoperatively and the latest, day 47. Distilled water and 10 mM EDTA treatments were the most efficient in retarding the appearance of PCO.

Original languageEnglish
Pages (from-to)2598-2605
Number of pages8
JournalJournal of Cataract and Refractive Surgery
Volume30
Issue number12
DOIs
StatePublished - Dec 1 2004

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Capsule Opacification
Hyaluronic Acid
Edetic Acid
Fluorouracil
Acetic Acid
Rabbits
Pharmaceutical Preparations
Water
Salts
Lenses
Capsulorhexis
Biophysics
Crystalline Lens
Phacoemulsification
Hardness
Mitomycin
Capsules
Medicine
Weights and Measures
Equipment and Supplies

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Efficacy of various drugs in the prevention of posterior capsule opacification : Experimental study of rabbit eyes. / Fernandez, Viviana; Fragoso, Miryam A.; Billotte, Christian; Lamar, Peggy; Orozco, Marcia A.; Dubovy, Sander; Willcox, Mark; Parel, Jean-Marie A.

In: Journal of Cataract and Refractive Surgery, Vol. 30, No. 12, 01.12.2004, p. 2598-2605.

Research output: Contribution to journalArticle

Fernandez, Viviana ; Fragoso, Miryam A. ; Billotte, Christian ; Lamar, Peggy ; Orozco, Marcia A. ; Dubovy, Sander ; Willcox, Mark ; Parel, Jean-Marie A. / Efficacy of various drugs in the prevention of posterior capsule opacification : Experimental study of rabbit eyes. In: Journal of Cataract and Refractive Surgery. 2004 ; Vol. 30, No. 12. pp. 2598-2605.
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