Efficacy of multivalent adenovirus-based vaccine against simian immunodeficiency virus challenge

Danilo R. Casimiro, Kara Cox, Aimin Tang, Kara J. Sykes, Meizhen Feng, Fubao Wang, Andrew Bett, William A. Schleif, Xiaoping Liang, Jessica Flynn, Timothy W. Tobery, Keith Wilson, Adam Finnefrock, Lingyi Huang, Salvatore Vitelli, Jing Lin, Deepa Patel, Mary Ellen Davies, Gwendolyn J. Heidecker, Daniel C. FreedSheri Dubey, David H. O'Connor, David Watkins, Zhi Qiang Zhang, John W. Shiver

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The prophylactic efficacies of several multivalent replication-incompetent adenovirus serotype 5 (Ad5) vaccines were examined in rhesus macaques using an intrarectal high-dose simian immunodeficiency virus SIVmac239 challenge model. Cohorts of Mamu-A*01+/B*17- Indian rhesus macaques were immunized with one of several combinations of Ad5 vectors expressing Gag, Pol, Nef, and Env gp140; for comparison, a Mamu-A*01 + cohort was immunized using the Ad5 vector alone. There was no sign of immunological interference between antigens in the immunized animals. In general, expansion of the antigen breadth resulted in more favorable virological outcomes. In particular, the order of efficacy trended as follows: Gag/Pol/Nef/Env ≈ Gag/Pol > Gag ≈ Gag/Pol/Nef > Nef. However, the precision in ranking the vaccines based on the study results may be limited by the cohort size, and as such, may warrant additional testing. The implications of these results in light of the recent discouraging results of the phase IIb study of the trivalent Ad5 HIV-1 vaccine are discussed.

Original languageEnglish
Pages (from-to)2996-3003
Number of pages8
JournalJournal of Virology
Volume84
Issue number6
DOIs
StatePublished - Mar 1 2010
Externally publishedYes

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Adenovirus Vaccines
Simian immunodeficiency virus
Simian Immunodeficiency Virus
Adenoviridae
serotypes
vaccines
Macaca mulatta
Vaccines
antigens
Antigens
AIDS Vaccines
Human immunodeficiency virus 1
crossover interference
HIV-1
Serogroup
dosage
animals
testing

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Casimiro, D. R., Cox, K., Tang, A., Sykes, K. J., Feng, M., Wang, F., ... Shiver, J. W. (2010). Efficacy of multivalent adenovirus-based vaccine against simian immunodeficiency virus challenge. Journal of Virology, 84(6), 2996-3003. https://doi.org/10.1128/JVI.00969-09

Efficacy of multivalent adenovirus-based vaccine against simian immunodeficiency virus challenge. / Casimiro, Danilo R.; Cox, Kara; Tang, Aimin; Sykes, Kara J.; Feng, Meizhen; Wang, Fubao; Bett, Andrew; Schleif, William A.; Liang, Xiaoping; Flynn, Jessica; Tobery, Timothy W.; Wilson, Keith; Finnefrock, Adam; Huang, Lingyi; Vitelli, Salvatore; Lin, Jing; Patel, Deepa; Davies, Mary Ellen; Heidecker, Gwendolyn J.; Freed, Daniel C.; Dubey, Sheri; O'Connor, David H.; Watkins, David; Zhang, Zhi Qiang; Shiver, John W.

In: Journal of Virology, Vol. 84, No. 6, 01.03.2010, p. 2996-3003.

Research output: Contribution to journalArticle

Casimiro, DR, Cox, K, Tang, A, Sykes, KJ, Feng, M, Wang, F, Bett, A, Schleif, WA, Liang, X, Flynn, J, Tobery, TW, Wilson, K, Finnefrock, A, Huang, L, Vitelli, S, Lin, J, Patel, D, Davies, ME, Heidecker, GJ, Freed, DC, Dubey, S, O'Connor, DH, Watkins, D, Zhang, ZQ & Shiver, JW 2010, 'Efficacy of multivalent adenovirus-based vaccine against simian immunodeficiency virus challenge', Journal of Virology, vol. 84, no. 6, pp. 2996-3003. https://doi.org/10.1128/JVI.00969-09
Casimiro, Danilo R. ; Cox, Kara ; Tang, Aimin ; Sykes, Kara J. ; Feng, Meizhen ; Wang, Fubao ; Bett, Andrew ; Schleif, William A. ; Liang, Xiaoping ; Flynn, Jessica ; Tobery, Timothy W. ; Wilson, Keith ; Finnefrock, Adam ; Huang, Lingyi ; Vitelli, Salvatore ; Lin, Jing ; Patel, Deepa ; Davies, Mary Ellen ; Heidecker, Gwendolyn J. ; Freed, Daniel C. ; Dubey, Sheri ; O'Connor, David H. ; Watkins, David ; Zhang, Zhi Qiang ; Shiver, John W. / Efficacy of multivalent adenovirus-based vaccine against simian immunodeficiency virus challenge. In: Journal of Virology. 2010 ; Vol. 84, No. 6. pp. 2996-3003.
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