Abstract
Two trials were conducted to evaluate two gram-negative bacterial control strategies in batch cultures of the rotifer Brachionus plicatilis. In the first trial, rotifers at an initial density of 47/mL were cultured for 5 d and dosed with a 10-mg/L solution of either oxytetracycline or a commercial probiotic consisting principally of Bacillus and Lactobacillus. In the second trial, rotifers at an initial density of 140/mL were cultured for 4 d and dosed with 20-mg/L solutions of oxytetracycline or the probiotic. Rotifer density, egg counts, and egg: female ratios were determined daily. At the termination of trial 1 and on days 0, 2, and 4 of trial 2, counts of bacterial colonies were conducted to estimate bacterial loads in the rotifer culture water. Oxytetracycline was an effective antimicrobial and enhanced rotifer production in the higher-density culture but had only a marginal effect on production in the lower-density culture. Conversely, inoculation of the low-density culture with the probiotic had no effect on rotifer production. Inoculation of the high-density culture adversely affected rotifer production by increasing mortality and reproductive inhibition.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 343-349 |
| Number of pages | 7 |
| Journal | North American Journal of Aquaculture |
| Volume | 73 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2011 |
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ASJC Scopus subject areas
- Aquatic Science
Cite this
Efficacy of a commercial probiotic relative to oxytetracycline as gram-negative bacterial control agents in a rotifer (Brachionus plicatihs) batch culture. / Rotman, Federico J.; Riche, Marty; Van Wyk, Peter; Benetti, Daniel D.
In: North American Journal of Aquaculture, Vol. 73, No. 3, 2011, p. 343-349.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Efficacy of a commercial probiotic relative to oxytetracycline as gram-negative bacterial control agents in a rotifer (Brachionus plicatihs) batch culture
AU - Rotman, Federico J.
AU - Riche, Marty
AU - Van Wyk, Peter
AU - Benetti, Daniel D
PY - 2011
Y1 - 2011
N2 - Two trials were conducted to evaluate two gram-negative bacterial control strategies in batch cultures of the rotifer Brachionus plicatilis. In the first trial, rotifers at an initial density of 47/mL were cultured for 5 d and dosed with a 10-mg/L solution of either oxytetracycline or a commercial probiotic consisting principally of Bacillus and Lactobacillus. In the second trial, rotifers at an initial density of 140/mL were cultured for 4 d and dosed with 20-mg/L solutions of oxytetracycline or the probiotic. Rotifer density, egg counts, and egg: female ratios were determined daily. At the termination of trial 1 and on days 0, 2, and 4 of trial 2, counts of bacterial colonies were conducted to estimate bacterial loads in the rotifer culture water. Oxytetracycline was an effective antimicrobial and enhanced rotifer production in the higher-density culture but had only a marginal effect on production in the lower-density culture. Conversely, inoculation of the low-density culture with the probiotic had no effect on rotifer production. Inoculation of the high-density culture adversely affected rotifer production by increasing mortality and reproductive inhibition.
AB - Two trials were conducted to evaluate two gram-negative bacterial control strategies in batch cultures of the rotifer Brachionus plicatilis. In the first trial, rotifers at an initial density of 47/mL were cultured for 5 d and dosed with a 10-mg/L solution of either oxytetracycline or a commercial probiotic consisting principally of Bacillus and Lactobacillus. In the second trial, rotifers at an initial density of 140/mL were cultured for 4 d and dosed with 20-mg/L solutions of oxytetracycline or the probiotic. Rotifer density, egg counts, and egg: female ratios were determined daily. At the termination of trial 1 and on days 0, 2, and 4 of trial 2, counts of bacterial colonies were conducted to estimate bacterial loads in the rotifer culture water. Oxytetracycline was an effective antimicrobial and enhanced rotifer production in the higher-density culture but had only a marginal effect on production in the lower-density culture. Conversely, inoculation of the low-density culture with the probiotic had no effect on rotifer production. Inoculation of the high-density culture adversely affected rotifer production by increasing mortality and reproductive inhibition.
UR - http://www.scopus.com/inward/record.url?scp=84977613179&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84977613179&partnerID=8YFLogxK
U2 - 10.1080/15222055.2011.603968
DO - 10.1080/15222055.2011.603968
M3 - Article
AN - SCOPUS:84977613179
VL - 73
SP - 343
EP - 349
JO - North American Journal of Aquaculture
JF - North American Journal of Aquaculture
SN - 1522-2055
IS - 3
ER -