Effects of tyrosine kinase inhibitor on α1B-AR-induced Ca2+ influx involved in hTrp3 protein

Xiao Ru Yang, Yong Yuan Guan, Qin Ying Qiu, Hua He, Jin Liang Li

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

AIM: To investigate the role of Trp3 in the Ca2+ influx induced by α1B-AR in HEK293 cells and the effect of tyrosine kinase on it. METHODS: With lipofect AMINE2000 reagent, hTrp3 cDNA was transfected to HEK293 cells and α1B-HEK293 cells respectively. The expression of Trp3 was examined by Western blot. With Fura-2/AM spectrophoto- fluorometry, Ca2+ influx was determined. RESULTS: HTrp3 was expressed endogenously in HEK293 cells, and the expression increased in hTrp3-transfected-cells. Compared with untransfected cells, transfection of hTrp3 cDNA increased Ca2+ influx induced by α1B-AR (P<0.01) without any change in thapsigargin-induced Ca2+ influx (P>0.05). 5-30 μmol · L-1 genistein inhibited Ca2+ influx induced by α1B-AR in hTrp3 cDNA -transfected cells and the maximum inhibitory rate was (75.2 ± 12.6)%. CONCLUSION: Transfection of hTrp3 cDNA increased Ca2+ influx induced by α1B-AR in HEK293 cells. This process was regulated by tyrosine kinase.

Original languageEnglish (US)
Pages (from-to)178-182
Number of pages5
JournalChinese Pharmacological Bulletin
Volume18
Issue number2
StatePublished - Jan 1 2002

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Keywords

  • α-AR
  • Genistein
  • ROC
  • Trp
  • Tyrosine kinase

ASJC Scopus subject areas

  • Pharmacology

Cite this

Yang, X. R., Guan, Y. Y., Qiu, Q. Y., He, H., & Li, J. L. (2002). Effects of tyrosine kinase inhibitor on α1B-AR-induced Ca2+ influx involved in hTrp3 protein. Chinese Pharmacological Bulletin, 18(2), 178-182.