AIM: To investigate the role of Trp3 in the Ca2+ influx induced by α1B-AR in HEK293 cells and the effect of tyrosine kinase on it. METHODS: With lipofect AMINE2000 reagent, hTrp3 cDNA was transfected to HEK293 cells and α1B-HEK293 cells respectively. The expression of Trp3 was examined by Western blot. With Fura-2/AM spectrophoto- fluorometry, Ca2+ influx was determined. RESULTS: HTrp3 was expressed endogenously in HEK293 cells, and the expression increased in hTrp3-transfected-cells. Compared with untransfected cells, transfection of hTrp3 cDNA increased Ca2+ influx induced by α1B-AR (P<0.01) without any change in thapsigargin-induced Ca2+ influx (P>0.05). 5-30 μmol · L-1 genistein inhibited Ca2+ influx induced by α1B-AR in hTrp3 cDNA -transfected cells and the maximum inhibitory rate was (75.2 ± 12.6)%. CONCLUSION: Transfection of hTrp3 cDNA increased Ca2+ influx induced by α1B-AR in HEK293 cells. This process was regulated by tyrosine kinase.
|Original language||English (US)|
|Number of pages||5|
|Journal||Chinese Pharmacological Bulletin|
|State||Published - Jan 1 2002|
- Tyrosine kinase
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