Effects of temperature, nucleotides and sodium molybdate on activation and DNA binding of estrogen, glucocorticoid, progesterone and androgen receptors in MCF-7 human cancer cells

Ming Ta Chong, Marc E. Lippman

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

We studied the effects of temperature, ribonucleotides and sodium molybdate on the activation and DNA cellulose binding of estrogen, glucocorticoid, progesterone and androgen receptor complexes in MCF-7 cells. Using DNA cellulose binding as a measure of receptor activation, we found that ribonucleotides activated all four of these receptor complexes. Temperature also activated glucocorticoid receptor complexes efficiently but activated progesterone and androgen receptor complexes less well. Temperature did not activate estrogen receptor complexes. Sodium molybdate blocked either ATP or temperature induced activation of glucocorticoid, progesterone and androgen receptor complexes but only partially blocked estrogen activation. Sodium molybdate also prevented the formation of multiple forms of estrogen and glucocorticoid receptor complexes seen on DEAE cellulose and hydroxylapatite chromatography of crude cytosol. The mechanism by which ribonucleotide enhances and molybdate inhibits activation are discussed.

Original languageEnglish (US)
Pages (from-to)575-600
Number of pages26
JournalJournal of Receptors and Signal Transduction
Volume2
Issue number5-6
DOIs
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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