Effects of storage temperature and time on clinical biochemical parameters from rat serum

Carolyn Cray, Marilyn Rodriguez, Julia Zaias, Norman H. Altaian

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Serum is often frozen and banked for analysis at a later date. This study assessed the stability of 17 analytes in rat serum during refrigeration at 4 °C and extended storage at -20 °C (frost-free and nonfrost-free freezers) and -70 °C. Samples were analyzed by using an automated dry-slide chemistry analyzer at time 0 and then stored as aliquots for analysis at time points including day 7,30, 90, and 360. After 7 d of refrigeration, only creatine kinase activity had varied by more than 10% of the starting value. Freezing at -70 °C was clearly superior to -20 °C where changes were observed in C0 2 as early as day 30 and alanine aminotransferase as early as day 90. Samples stored in frost-free and nonfrost-free -20 °C freezers did not differ significantly through day 90. Factors such as storage time and temperature should be considered when designing any retrospective study.

Original languageEnglish
Pages (from-to)202-204
Number of pages3
JournalJournal of the American Association for Laboratory Animal Science
Volume48
Issue number2
StatePublished - Mar 1 2009

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freezers
refrigeration
frost
storage temperature
storage time
rats
creatine kinase
alanine transaminase
retrospective studies
freezing
chemistry
sampling

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Effects of storage temperature and time on clinical biochemical parameters from rat serum. / Cray, Carolyn; Rodriguez, Marilyn; Zaias, Julia; Altaian, Norman H.

In: Journal of the American Association for Laboratory Animal Science, Vol. 48, No. 2, 01.03.2009, p. 202-204.

Research output: Contribution to journalArticle

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