Effects of stomatal density and leaf water content on the 18O enrichment of leaf water

Leticia Larcher, Ikuko Hara-Nishimura, Leonel Sternberg

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Leaf water isotopic composition is imprinted in several biomarkers of interest and it is imperative that we understand the isotopic enrichment of leaf water. Here, we test the effect of stomatal density and leaf water content on the oxygen isotopic composition of leaf water in transgenic Arabidopsis plants expressing different stomatal densities, and several other species showing a range of stomatal density. We grew Arabidopsis plants hydroponically and collected other species in the field. Stomatal density and leaf water content were determined for each plant. We measured transpiration and extracted leaf water for isotopic determination. Using these measurements and the current leaf water isotope model, we calculated several of the parameters related to leaf water isotopic enrichment. High stomatal density promoted leaf water isotope enrichment. No conclusion, however, can be drawn regarding the effect of leaf water content on leaf water isotope enrichment. Factors such as transpiration might mask the effect of stomatal density on leaf water isotopic enrichment. We propose a method by which stomatal density can be incorporated in the current Peclet model of leaf water isotope enrichment. These findings have important applications in the use of plant-based metabolic proxies in paleoclimate studies.

Original languageEnglish
Pages (from-to)141-151
Number of pages11
JournalNew Phytologist
Volume206
Issue number1
DOIs
StatePublished - Apr 1 2015

Fingerprint

water content
Water
leaves
water
Isotopes
isotopes
Arabidopsis
transpiration
Genetically Modified Plants
Proxy
Masks
biomarkers
Biomarkers
genetically modified organisms
Oxygen
oxygen

Keywords

  • Arabidopsis
  • Leaf water
  • Leaf water content
  • Oxygen isotope ratios
  • Stomatal density

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Effects of stomatal density and leaf water content on the 18O enrichment of leaf water. / Larcher, Leticia; Hara-Nishimura, Ikuko; Sternberg, Leonel.

In: New Phytologist, Vol. 206, No. 1, 01.04.2015, p. 141-151.

Research output: Contribution to journalArticle

Larcher, Leticia ; Hara-Nishimura, Ikuko ; Sternberg, Leonel. / Effects of stomatal density and leaf water content on the 18O enrichment of leaf water. In: New Phytologist. 2015 ; Vol. 206, No. 1. pp. 141-151.
@article{c419feca0bfc4012997c297b32aa8de9,
title = "Effects of stomatal density and leaf water content on the 18O enrichment of leaf water",
abstract = "Leaf water isotopic composition is imprinted in several biomarkers of interest and it is imperative that we understand the isotopic enrichment of leaf water. Here, we test the effect of stomatal density and leaf water content on the oxygen isotopic composition of leaf water in transgenic Arabidopsis plants expressing different stomatal densities, and several other species showing a range of stomatal density. We grew Arabidopsis plants hydroponically and collected other species in the field. Stomatal density and leaf water content were determined for each plant. We measured transpiration and extracted leaf water for isotopic determination. Using these measurements and the current leaf water isotope model, we calculated several of the parameters related to leaf water isotopic enrichment. High stomatal density promoted leaf water isotope enrichment. No conclusion, however, can be drawn regarding the effect of leaf water content on leaf water isotope enrichment. Factors such as transpiration might mask the effect of stomatal density on leaf water isotopic enrichment. We propose a method by which stomatal density can be incorporated in the current Peclet model of leaf water isotope enrichment. These findings have important applications in the use of plant-based metabolic proxies in paleoclimate studies.",
keywords = "Arabidopsis, Leaf water, Leaf water content, Oxygen isotope ratios, Stomatal density",
author = "Leticia Larcher and Ikuko Hara-Nishimura and Leonel Sternberg",
year = "2015",
month = "4",
day = "1",
doi = "10.1111/nph.13154",
language = "English",
volume = "206",
pages = "141--151",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Effects of stomatal density and leaf water content on the 18O enrichment of leaf water

AU - Larcher, Leticia

AU - Hara-Nishimura, Ikuko

AU - Sternberg, Leonel

PY - 2015/4/1

Y1 - 2015/4/1

N2 - Leaf water isotopic composition is imprinted in several biomarkers of interest and it is imperative that we understand the isotopic enrichment of leaf water. Here, we test the effect of stomatal density and leaf water content on the oxygen isotopic composition of leaf water in transgenic Arabidopsis plants expressing different stomatal densities, and several other species showing a range of stomatal density. We grew Arabidopsis plants hydroponically and collected other species in the field. Stomatal density and leaf water content were determined for each plant. We measured transpiration and extracted leaf water for isotopic determination. Using these measurements and the current leaf water isotope model, we calculated several of the parameters related to leaf water isotopic enrichment. High stomatal density promoted leaf water isotope enrichment. No conclusion, however, can be drawn regarding the effect of leaf water content on leaf water isotope enrichment. Factors such as transpiration might mask the effect of stomatal density on leaf water isotopic enrichment. We propose a method by which stomatal density can be incorporated in the current Peclet model of leaf water isotope enrichment. These findings have important applications in the use of plant-based metabolic proxies in paleoclimate studies.

AB - Leaf water isotopic composition is imprinted in several biomarkers of interest and it is imperative that we understand the isotopic enrichment of leaf water. Here, we test the effect of stomatal density and leaf water content on the oxygen isotopic composition of leaf water in transgenic Arabidopsis plants expressing different stomatal densities, and several other species showing a range of stomatal density. We grew Arabidopsis plants hydroponically and collected other species in the field. Stomatal density and leaf water content were determined for each plant. We measured transpiration and extracted leaf water for isotopic determination. Using these measurements and the current leaf water isotope model, we calculated several of the parameters related to leaf water isotopic enrichment. High stomatal density promoted leaf water isotope enrichment. No conclusion, however, can be drawn regarding the effect of leaf water content on leaf water isotope enrichment. Factors such as transpiration might mask the effect of stomatal density on leaf water isotopic enrichment. We propose a method by which stomatal density can be incorporated in the current Peclet model of leaf water isotope enrichment. These findings have important applications in the use of plant-based metabolic proxies in paleoclimate studies.

KW - Arabidopsis

KW - Leaf water

KW - Leaf water content

KW - Oxygen isotope ratios

KW - Stomatal density

UR - http://www.scopus.com/inward/record.url?scp=84923269572&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84923269572&partnerID=8YFLogxK

U2 - 10.1111/nph.13154

DO - 10.1111/nph.13154

M3 - Article

C2 - 25408145

AN - SCOPUS:84923269572

VL - 206

SP - 141

EP - 151

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 1

ER -