Effects of Schwann cells and donor antigen on long-nerve allograft regeneration

Michael J. Brenner, James B. Lowe, Ida K. Fox, Susan E. Mackinnon, Daniel A. Hunter, Michael D. Darcy, James R. Duncan, Patrick Wood, Thalachallour Mohanakumar

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Nerve allotransplantation has been used successfully in human subjects to restore function after traumatic nerve injury and avoid subsequent limb amputation. However, due to the morbidity associated with nonspecific immunosuppression, this reconstructive approach has been limited to patients with particularly severe nerve injuries. It would be desirable to broaden the indications for such procedures through development of less toxic antirejection therapies. A miniature swine model of nerve transplantation was used to investigate the effects of preoperative ultraviolet-B (UV-B)-irradiated donor alloantigen portal venous infusion and injection of cultured major histocompatibility complex (MHC)-matched Schwann cells into the nerve graft. The transplanted ulnar nerves were harvested at 20 weeks. Histomorphometry showed marked enhancement in nerve regeneration through allografts injected with Schwann cells. Serial mixed lymphocyte assays demonstrated suppression of the recipient immune response to the donor antigen after pretreatment, but no additional neuroregenerative effect of donor alloantigen pretreatment.

Original languageEnglish
Pages (from-to)61-70
Number of pages10
JournalMicrosurgery
Volume25
Issue number1
DOIs
StatePublished - Feb 17 2005

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Nerve Regeneration
Isoantigens
Schwann Cells
Immunosuppression
Allografts
Miniature Swine
Antigens
Ulnar Nerve
Poisons
Wounds and Injuries
Major Histocompatibility Complex
Amputation
Extremities
Transplantation
Lymphocytes
Morbidity
Transplants
Injections

ASJC Scopus subject areas

  • Surgery

Cite this

Brenner, M. J., Lowe, J. B., Fox, I. K., Mackinnon, S. E., Hunter, D. A., Darcy, M. D., ... Mohanakumar, T. (2005). Effects of Schwann cells and donor antigen on long-nerve allograft regeneration. Microsurgery, 25(1), 61-70. https://doi.org/10.1002/micr.20083

Effects of Schwann cells and donor antigen on long-nerve allograft regeneration. / Brenner, Michael J.; Lowe, James B.; Fox, Ida K.; Mackinnon, Susan E.; Hunter, Daniel A.; Darcy, Michael D.; Duncan, James R.; Wood, Patrick; Mohanakumar, Thalachallour.

In: Microsurgery, Vol. 25, No. 1, 17.02.2005, p. 61-70.

Research output: Contribution to journalArticle

Brenner, MJ, Lowe, JB, Fox, IK, Mackinnon, SE, Hunter, DA, Darcy, MD, Duncan, JR, Wood, P & Mohanakumar, T 2005, 'Effects of Schwann cells and donor antigen on long-nerve allograft regeneration', Microsurgery, vol. 25, no. 1, pp. 61-70. https://doi.org/10.1002/micr.20083
Brenner MJ, Lowe JB, Fox IK, Mackinnon SE, Hunter DA, Darcy MD et al. Effects of Schwann cells and donor antigen on long-nerve allograft regeneration. Microsurgery. 2005 Feb 17;25(1):61-70. https://doi.org/10.1002/micr.20083
Brenner, Michael J. ; Lowe, James B. ; Fox, Ida K. ; Mackinnon, Susan E. ; Hunter, Daniel A. ; Darcy, Michael D. ; Duncan, James R. ; Wood, Patrick ; Mohanakumar, Thalachallour. / Effects of Schwann cells and donor antigen on long-nerve allograft regeneration. In: Microsurgery. 2005 ; Vol. 25, No. 1. pp. 61-70.
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