Effects of salinity on copper accumulation in the common killifish (Fundulus heteroclitus)

Jonathan Blanchard, Martin Grosell

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Results of laboratory and field studies have demonstrated that salinity influences the accumulation of copper. The present study is, to our knowledge, the first to examine the effect of salinity on copper accumulation in a teleost fish across a comprehensive range of salinity from freshwater to seawater. This was done in an effort to identify potential target tissues and differences in chemical interactions across salinities that will aid in the development of a seawater biotic ligand model (BLM) for copper. Killifish (Fundulus heteroclitus) were acclimated to five salinities (0, 5, 11, 22, and 28 ppt) and exposed to three copper concentrations (0 [nominal], 30, and 150 μg L-1), yielding 15 treatment groups. Fish from each group were sampled for tissue copper analysis at 0, 4, 12, and 30 d postexposure. Whole-body and liver accumulations were highest at lower salinities. The liver accounted for 57 to 86% of the whole-body copper even though it accounted for less than 4% of the body mass. Similarly, the gill accumulated more copper at lower salinities, whereas the intestine generally accumulated more copper at higher salinities. Speciation calculations indicate that CuCO3 likely accounts for much of the accumulation, possibly with some contributions from CuOH+ and Cu(OH)2. The free ion, Cu2+, does not appear to be associated with copper accumulation. However, the differences in physiology and in the concentrations of competing cations across salinities suggest that speciation alone cannot explain accumulation. The present findings may have implications for future development of a BLM for saline environments by identifying potential target tissues.

Original languageEnglish
Pages (from-to)1403-1413
Number of pages11
JournalEnvironmental Toxicology and Chemistry
Volume24
Issue number6
DOIs
StatePublished - Jun 1 2005

Fingerprint

Fundulidae
Salinity
Copper
copper
salinity
Seawater
Tissue
Liver
Fish
ligand
Fishes
Ligands
seawater
effect
Physiology
fish
Fresh Water
teleost
body mass
Intestines

Keywords

  • Brackish water
  • Copper homeostasis
  • Inorganic speciation
  • Mummichog
  • Seawater

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Effects of salinity on copper accumulation in the common killifish (Fundulus heteroclitus). / Blanchard, Jonathan; Grosell, Martin.

In: Environmental Toxicology and Chemistry, Vol. 24, No. 6, 01.06.2005, p. 1403-1413.

Research output: Contribution to journalArticle

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