Effects of recombinant sea anemone cytotoxin on vascular smooth muscle cell proliferation in rats

Ping Ouyang, Xiao Yu Jiang, Wen Li Yang, An Long Xu

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To observe the effects of recombined sea anemone cytotoxin (rSAC) on vascular smooth muscle cell proliferation in rats rSAC. METHODS: Aortic smooth muscle cells (ASMCs) of rats were cultured in vitro and stimulated with rSAC of different concentrations, and were observed in comparison with the control group. The ratio of cell proliferation was determined with non-radioactive MTS/PES assay. RESULTS: The ratio of cell proliferation rate was 0.802+/-0.055 in the control group, and was 0.115+/-0.014, 0.213+/-0.016, 0.335+/-0.097, 0.663+/-0.060, 0.678+/-0.129, and 0.738+/-0.072 in rSAC groups corresponding to rSAC concentrations of 100, 10, 1 mg/ml and 100, 10, 1 ng/ml respectively. It was shown that rSAC significantly inhibited the cell proliferation when the concentration used was above 100 ng/mg (P<0.05), in a dose-dependent manner as compared with the control group (P<0.05). CONCLUSION: rSAC can significantly inhibit the vascular smooth muscle cell proliferation in rats.

Original languageEnglish (US)
Pages (from-to)994-995
Number of pages2
JournalDi 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
Volume22
Issue number11
StatePublished - Nov 2002
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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