Effects of recombinant Sagartia rosea cytolysin and Lapemis hardwickii phospholipase A2 on adventitial fibroblasts proliferation.

Ping Ouyang, Xiao yu Jiang, Wen li Yang, Wen yan Lai, An long Xu

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To observe the effects of recombinant Sagartia rosea cytolysin (rSrc) and Lapemis hardwickii phospholipase A2(rPLA2) on adventitial fibroblasts proliferation. METHODS: NIH-3T3 cells were cultured and treated with rSrc and rPLA2 at different concentrations for observation of cell proliferation using non-radioactive MTS/PES assay in comparison with the control group. RESULTS: The ratio of cell proliferation was 0.840+/-0.061 in the control group, and was 0.263+/-0.044, 0.418+/-0.054, 0.605+/-0.063, 0.772+/-0.054 and 0.906+/-0.072 in rSrc groups corresponding to rSrc concentrations of 100, 10, 1 microg/ml and 100, 10 ng/ml respectively. rSrc was found to significantly inhibit fibroblast proliferation in a dose-dependent manner when the concentration used was above 1 microg/ml (P<0.05), as compared with the control group (P<0.05). The ratio of cell proliferation was 0.498+/-0.076, 0.937+/-0.112 and 0.978+/-0.145 in rPLA2 groups corresponding to rPLA2 concentrations of 100, 10, 1 microg/ml respectively, indicating that rPLA2 also significantly inhibited fibroblast proliferation at the concentration of 100 microg/ml (P<0.05). CONCLUSION: rSrc and rPLA2 can both significantly inhibit adventitial fibroblast proliferation.

Original languageEnglish (US)
Pages (from-to)158-160
Number of pages3
JournalDi 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
Volume24
Issue number2
StatePublished - Feb 2004
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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