Effects of psoralen on the structural integrity of cultured osteoblasts - Phase contrast, immunofluorescent, and electronmicroscopic evaluation

George I. Malinin, William J. Vornberger, Francis J. Hornicek, Theodore Malinin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The 72-h dark interaction of cultured osteoblasts with 0.5-1.0 μg/ml 8-methoxypsoralen (8-MOP) resulted in the accumulation of cytoplasmic lipid droplets (steatosis) in target cells. These methanol-extractable lipid droplets and 8-MOP, however, had no microscopically detectable effect on the organization of α-actin and β-tubulin-containing filaments. On the ultrastructural level, psoralen effects ranged from negligible to unambiguous structural alterations of target cells. The latter consisted of blebbing, segmental deletions of the nuclear envelope, and structural unraveling of nucleoli. Moreover, the dilatation of the rough endoplasmic reticulum, the decrease in the number of ribosomes, and the extensive vacuolation of cytoplasm constituted yet another hallmark of the 8-MOP effect in the absence of light. Whereas psoralen has induced a number of structural alterations in some, but not in all osteoblasts, more than 95% of the target cells have, nonetheless, remained viable. Taken together, our results suggest that the dark reaction of psoralen with osteoblasts results mainly in transient structural alterations of affected cells.

Original languageEnglish
Pages (from-to)182-188
Number of pages7
JournalArchives of Toxicology
Volume70
Issue number3-4
DOIs
StatePublished - Jan 1 1996

Fingerprint

Methoxsalen
Ficusin
Osteoblasts
Structural integrity
Lipids
Tubulin
Methanol
Rough Endoplasmic Reticulum
Actins
Nuclear Envelope
Blister
Ribosomes
Dilatation
Cytoplasm
Light
Lipid Droplets

Keywords

  • Dark reaction
  • Microscopy
  • Osteoblasts
  • Psoralen
  • Structural alterations

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Effects of psoralen on the structural integrity of cultured osteoblasts - Phase contrast, immunofluorescent, and electronmicroscopic evaluation. / Malinin, George I.; Vornberger, William J.; Hornicek, Francis J.; Malinin, Theodore.

In: Archives of Toxicology, Vol. 70, No. 3-4, 01.01.1996, p. 182-188.

Research output: Contribution to journalArticle

Malinin, George I. ; Vornberger, William J. ; Hornicek, Francis J. ; Malinin, Theodore. / Effects of psoralen on the structural integrity of cultured osteoblasts - Phase contrast, immunofluorescent, and electronmicroscopic evaluation. In: Archives of Toxicology. 1996 ; Vol. 70, No. 3-4. pp. 182-188.
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