Effects of procaine amide on the electrophysiologic properties of the canine ventricular conducting system

M. R. Rosen, C. Merker, H. Gelband, B. F. Hoffman

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Microelectrode techniques were used to study effects of procainamide (PA), 10 to 90 μg/ml, on electrophysiological properties of canine Purkinje fibers (PF) and ventricular muscle perfused with Tyrode's solution containing 2.5, 4.0 or 5.5 mM KCl. Automaticity of spontaneously beating PF decreased within 40 min of perfusion with [PA] = 10 μg/ml; this was the only consistent electrophysiological change at [PA] < 30 μg/ml. After 30 min of perfusion with [PA] = 30 μg/ml, action potential amplitude and maximal slope of phase 0 decreased, and conduction throughout the ventricular conducting system was slowed, especially that in ventricular muscle. The voltage time course of repolarization was altered in such a way that action potentials dissimilar in duration, recorded from sites proximal to, distal to and at the area of maximum action potential duration became similar. Simultaneously action potential duration measured to full repolarization and effective refractory periods were prolonged. Changes were most prominent at [K+]0 = 2.5 and least apparent at [K+]0 = 5.5 mM. At [PA] = 90 μg/ml the aforementioned changes were accentuated.

Original languageEnglish
Pages (from-to)438-446
Number of pages9
JournalJournal of Pharmacology and Experimental Therapeutics
Volume185
Issue number3
StatePublished - Dec 1 1973
Externally publishedYes

Fingerprint

Procainamide
Canidae
Action Potentials
Purkinje Fibers
Perfusion
Muscles
Microelectrodes

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effects of procaine amide on the electrophysiologic properties of the canine ventricular conducting system. / Rosen, M. R.; Merker, C.; Gelband, H.; Hoffman, B. F.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 185, No. 3, 01.12.1973, p. 438-446.

Research output: Contribution to journalArticle

@article{64caaba82be547fe9ed7e3cc90451613,
title = "Effects of procaine amide on the electrophysiologic properties of the canine ventricular conducting system",
abstract = "Microelectrode techniques were used to study effects of procainamide (PA), 10 to 90 μg/ml, on electrophysiological properties of canine Purkinje fibers (PF) and ventricular muscle perfused with Tyrode's solution containing 2.5, 4.0 or 5.5 mM KCl. Automaticity of spontaneously beating PF decreased within 40 min of perfusion with [PA] = 10 μg/ml; this was the only consistent electrophysiological change at [PA] < 30 μg/ml. After 30 min of perfusion with [PA] = 30 μg/ml, action potential amplitude and maximal slope of phase 0 decreased, and conduction throughout the ventricular conducting system was slowed, especially that in ventricular muscle. The voltage time course of repolarization was altered in such a way that action potentials dissimilar in duration, recorded from sites proximal to, distal to and at the area of maximum action potential duration became similar. Simultaneously action potential duration measured to full repolarization and effective refractory periods were prolonged. Changes were most prominent at [K+]0 = 2.5 and least apparent at [K+]0 = 5.5 mM. At [PA] = 90 μg/ml the aforementioned changes were accentuated.",
author = "Rosen, {M. R.} and C. Merker and H. Gelband and Hoffman, {B. F.}",
year = "1973",
month = "12",
day = "1",
language = "English",
volume = "185",
pages = "438--446",
journal = "Journal of Pharmacology and Experimental Therapeutics",
issn = "0022-3565",
publisher = "American Society for Pharmacology and Experimental Therapeutics",
number = "3",

}

TY - JOUR

T1 - Effects of procaine amide on the electrophysiologic properties of the canine ventricular conducting system

AU - Rosen, M. R.

AU - Merker, C.

AU - Gelband, H.

AU - Hoffman, B. F.

PY - 1973/12/1

Y1 - 1973/12/1

N2 - Microelectrode techniques were used to study effects of procainamide (PA), 10 to 90 μg/ml, on electrophysiological properties of canine Purkinje fibers (PF) and ventricular muscle perfused with Tyrode's solution containing 2.5, 4.0 or 5.5 mM KCl. Automaticity of spontaneously beating PF decreased within 40 min of perfusion with [PA] = 10 μg/ml; this was the only consistent electrophysiological change at [PA] < 30 μg/ml. After 30 min of perfusion with [PA] = 30 μg/ml, action potential amplitude and maximal slope of phase 0 decreased, and conduction throughout the ventricular conducting system was slowed, especially that in ventricular muscle. The voltage time course of repolarization was altered in such a way that action potentials dissimilar in duration, recorded from sites proximal to, distal to and at the area of maximum action potential duration became similar. Simultaneously action potential duration measured to full repolarization and effective refractory periods were prolonged. Changes were most prominent at [K+]0 = 2.5 and least apparent at [K+]0 = 5.5 mM. At [PA] = 90 μg/ml the aforementioned changes were accentuated.

AB - Microelectrode techniques were used to study effects of procainamide (PA), 10 to 90 μg/ml, on electrophysiological properties of canine Purkinje fibers (PF) and ventricular muscle perfused with Tyrode's solution containing 2.5, 4.0 or 5.5 mM KCl. Automaticity of spontaneously beating PF decreased within 40 min of perfusion with [PA] = 10 μg/ml; this was the only consistent electrophysiological change at [PA] < 30 μg/ml. After 30 min of perfusion with [PA] = 30 μg/ml, action potential amplitude and maximal slope of phase 0 decreased, and conduction throughout the ventricular conducting system was slowed, especially that in ventricular muscle. The voltage time course of repolarization was altered in such a way that action potentials dissimilar in duration, recorded from sites proximal to, distal to and at the area of maximum action potential duration became similar. Simultaneously action potential duration measured to full repolarization and effective refractory periods were prolonged. Changes were most prominent at [K+]0 = 2.5 and least apparent at [K+]0 = 5.5 mM. At [PA] = 90 μg/ml the aforementioned changes were accentuated.

UR - http://www.scopus.com/inward/record.url?scp=0015750367&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015750367&partnerID=8YFLogxK

M3 - Article

C2 - 4712643

AN - SCOPUS:0015750367

VL - 185

SP - 438

EP - 446

JO - Journal of Pharmacology and Experimental Therapeutics

JF - Journal of Pharmacology and Experimental Therapeutics

SN - 0022-3565

IS - 3

ER -