Effects of PPAR-delta agonist and zinc on vaginal smooth muscle cells collagen and tropoelastin production

Peter Takacs, Yanping Zhang, Keith Candiotti, Sindy Jaramillo, Carlos A. Medina

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Introduction and hypothesis: The objective was to measure the effects of peroxisome proliferator-activated receptor delta (PPAR-delta) agonist and zinc sulfate (ZS) on vaginal smooth muscle cell (SMC) proliferation, tropoelastin production, and collagen production. Methods: SMC cultures were performed from vaginal wall biopsies and were incubated with ZS and PPAR-delta agonist GW501516 (GW). Cell proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay. Supernatants and cell lysates were collected. Tropoelastin production was measured by Fastin Elastin Assay and collagen was measured with Sircol Collagen Assay. Results: SMC proliferation was similar with 20 μM ZS or 10 nM GW compared to control. Tropoelastin production was significantly increased by 20 μM ZS and by 10 nM GW. Cell culture surface deposited elastin production was significantly increased by 20 μM ZS and by the combination of 20 μM ZS with 10 nM GW, and collagen production was significantly increased by 10 nM GW and by the combination of 20 μM ZS with 10 nM GW. Conclusions: PPAR-delta agonist and ZS increases vaginal SMC tropoelastin and collagen production.

Original languageEnglish (US)
Pages (from-to)1775-1779
Number of pages5
JournalInternational Urogynecology Journal and Pelvic Floor Dysfunction
Volume23
Issue number12
DOIs
StatePublished - Dec 1 2012

Keywords

  • Collagen
  • Elastin
  • Peroxisome proliferator-activated receptor delta
  • Vagina
  • Zinc

ASJC Scopus subject areas

  • Urology
  • Obstetrics and Gynecology

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