Effects of post-mortem delay on subunits of ionotropic glutamate receptors in human brain

Yuehua Wang, Emebet TesFaye, Robert P. Yasuda, Deborah C Mash, David M. Armstrong, Barry B. Wolfe

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The effect of post-mortem delay on the stability of the protein subunits that combine to form NMDA and AMPA type glutamate receptors has been assessed in samples of human brain tissue. While most of the subunits (i.e. GluR1, GluR2/3, GluR4, NR1) appear to be stable for up to 18 h post-mortem, the NR2A and NR2B subunits appear to be proteolyzed rapidly following death. These results are consistent with the concept that the proteolytic products of NR2A and NR2B, although at smaller molecular sizes than the full-length protein, are all identifiable on Western blots. Thus, a method is proposed that allows for the estimation of the levels of these labile proteins even in samples obtained up to 18 h post-mortem. Using this method we have estimated the levels of all AMPA and NMDA receptor subunits in selected (i.e. hippocampus, frontal and entorhinal cortex) brain tissue samples obtained from control patients and patients who have died with Alzheimer's disease. Modest decreases in NMDA receptor subunits NR1, NR2A, and NR2B were found in the hippocampus and in frontal cortex while little or no change in any of these subunits were documented in entorhinal cortex. Subunits for AMPA receptors (GluR1, GluR2/3, and GluR4) appeared to show a generalized decrease in all these tissues. As a surrogate marker for overall decreases due to generalized neuronal cell death, levels of neuron-specific enolase were measured in all tissues and were found to be nearly identical in control and Alzheimer's brains. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)123-131
Number of pages9
JournalMolecular Brain Research
Volume80
Issue number2
DOIs
StatePublished - Sep 15 2000

Fingerprint

Ionotropic Glutamate Receptors
Entorhinal Cortex
AMPA Receptors
Brain
Frontal Lobe
Hippocampus
Phosphopyruvate Hydratase
Protein Subunits
Glutamate Receptors
N-Methylaspartate
N-Methyl-D-Aspartate Receptors
Alzheimer Disease
Proteins
Cell Death
Biomarkers
Western Blotting

Keywords

  • Alzheimer's disease
  • Glutamate receptor
  • Human brain
  • Immunoblot
  • Post-mortem delay
  • Protein stability

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Wang, Y., TesFaye, E., Yasuda, R. P., Mash, D. C., Armstrong, D. M., & Wolfe, B. B. (2000). Effects of post-mortem delay on subunits of ionotropic glutamate receptors in human brain. Molecular Brain Research, 80(2), 123-131. https://doi.org/10.1016/S0169-328X(00)00111-X

Effects of post-mortem delay on subunits of ionotropic glutamate receptors in human brain. / Wang, Yuehua; TesFaye, Emebet; Yasuda, Robert P.; Mash, Deborah C; Armstrong, David M.; Wolfe, Barry B.

In: Molecular Brain Research, Vol. 80, No. 2, 15.09.2000, p. 123-131.

Research output: Contribution to journalArticle

Wang, Y, TesFaye, E, Yasuda, RP, Mash, DC, Armstrong, DM & Wolfe, BB 2000, 'Effects of post-mortem delay on subunits of ionotropic glutamate receptors in human brain', Molecular Brain Research, vol. 80, no. 2, pp. 123-131. https://doi.org/10.1016/S0169-328X(00)00111-X
Wang, Yuehua ; TesFaye, Emebet ; Yasuda, Robert P. ; Mash, Deborah C ; Armstrong, David M. ; Wolfe, Barry B. / Effects of post-mortem delay on subunits of ionotropic glutamate receptors in human brain. In: Molecular Brain Research. 2000 ; Vol. 80, No. 2. pp. 123-131.
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