Effects of Pancreas Cold Ischemia on Islet Function and Quality

A. Pileggi, M. M. Ribeiro, Anthony Richard Hogan, Ruth Molano, J. E. Embury, H. Ichii, L. Cobianchi, Alessia Fornoni, Camillo Ricordi, Ricardo Pastori

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Abstract

We used a rat model of pancreas cold preservation to assess its effects on islets. Glands were surgically retrieved and stored in University of Wisconsin (UW) solution for 3 hours (Short) or 18 hours (Long) cold ischemia time (CIT). Islet yield was significantly lower in the Long-CIT than the Short-CIT group, as well as islet recovery after overnight culture (P < .01). Islet cell viability after isolation was significantly reduced in the Long-CIT group (P < .05). Reversal of diabetes following transplantation of suboptimal islet grafts occurred earlier in the Short-CIT group than the Long-CIT. All animals in the Short-CIT group and 80% in the Long-CIT group achieved euglycemia. Freshly isolated islets showed a significant increase of JNK and p38 (P < .05) phosphorylation in Long-CIT compared with Short-CIT. Histopathological assessment of the pancreas showed a significantly higher injury score. Proteomic analysis of pancreatic tissue led to identification of 5 proteins consistently differentially expressed between Short-CIT and Long-CIT. Better understanding of the molecular pathways involved in this phenomenon will be of assistance in defining targeted interventions to improve organ use in the clinical arena.

Original languageEnglish
Pages (from-to)1808-1809
Number of pages2
JournalTransplantation Proceedings
Volume41
Issue number5
DOIs
StatePublished - Jun 1 2009

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Cold Ischemia
Pancreas
Islets of Langerhans Transplantation
Islets of Langerhans
Proteomics
Cell Survival

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Effects of Pancreas Cold Ischemia on Islet Function and Quality. / Pileggi, A.; Ribeiro, M. M.; Hogan, Anthony Richard; Molano, Ruth; Embury, J. E.; Ichii, H.; Cobianchi, L.; Fornoni, Alessia; Ricordi, Camillo; Pastori, Ricardo.

In: Transplantation Proceedings, Vol. 41, No. 5, 01.06.2009, p. 1808-1809.

Research output: Contribution to journalArticle

Pileggi, A. ; Ribeiro, M. M. ; Hogan, Anthony Richard ; Molano, Ruth ; Embury, J. E. ; Ichii, H. ; Cobianchi, L. ; Fornoni, Alessia ; Ricordi, Camillo ; Pastori, Ricardo. / Effects of Pancreas Cold Ischemia on Islet Function and Quality. In: Transplantation Proceedings. 2009 ; Vol. 41, No. 5. pp. 1808-1809.
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