Effects of NG-monomethyl-L-arginine and L-arginine on acetylcholine renal response

Vicente Lahera, Miguel G. Salom, Mary J. Fiksen-Olsen, Leopoldo Raij, J. Carlos Romero

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Intrarenal infusion of acetylcholine in meclofenamate-treated dogs significantly increased renal blood flow, diuresis, and natriuresis. Intrarenal infusions of either NG-monomethyl-L-arginine (inhibitor of endothelium-derived relaxing factor formation), or L-arginine (precursor of endothelium-derived relaxing factor formation) did not modify basal levels of those parameters. However, the infusion of NG-monomethyl-L-arginine inhibited the acetylcholine-induced increases in renal blood flow and diuresis without affecting natriuresis, which increased significantly. The infusion of L-arginine failed to further enhance hemodynamic and excretory effects elicited by acetylcholine. The concomitant infusion of L-arginine and NG-monomethyl-L-arginine did not change renal blood flow, urine flow, or sodium excretion rate. L-Arginine administration prevented the inhibitory effect of NG-monomethyl-L-arginine on acetylcholine-induced renal vasodilatation and diuresis. Glomerular filtration rate and mean arterial pressure did not change throughout the experiment. The results indicate that the vasodilatory and diuretic responses to intrarenal acetylcholine in meclofenamate-treated dogs are largely dependent on endothelium-derived relaxing factor.

Original languageEnglish
Pages (from-to)659-663
Number of pages5
JournalHypertension
Volume15
Issue number6 PART 1
StatePublished - Jun 1 1990
Externally publishedYes

Fingerprint

omega-N-Methylarginine
Acetylcholine
Arginine
Endothelium-Dependent Relaxing Factors
Renal Circulation
Diuresis
Kidney
Meclofenamic Acid
Natriuresis
Dogs
Glomerular Filtration Rate
Diuretics
Vasodilation
Arterial Pressure
Hemodynamics
Sodium
Urine

Keywords

  • Acetylcholine
  • Arginine
  • Endothelium
  • Hemodynamics
  • Renal function

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Lahera, V., Salom, M. G., Fiksen-Olsen, M. J., Raij, L., & Romero, J. C. (1990). Effects of NG-monomethyl-L-arginine and L-arginine on acetylcholine renal response. Hypertension, 15(6 PART 1), 659-663.

Effects of NG-monomethyl-L-arginine and L-arginine on acetylcholine renal response. / Lahera, Vicente; Salom, Miguel G.; Fiksen-Olsen, Mary J.; Raij, Leopoldo; Romero, J. Carlos.

In: Hypertension, Vol. 15, No. 6 PART 1, 01.06.1990, p. 659-663.

Research output: Contribution to journalArticle

Lahera, V, Salom, MG, Fiksen-Olsen, MJ, Raij, L & Romero, JC 1990, 'Effects of NG-monomethyl-L-arginine and L-arginine on acetylcholine renal response', Hypertension, vol. 15, no. 6 PART 1, pp. 659-663.
Lahera V, Salom MG, Fiksen-Olsen MJ, Raij L, Romero JC. Effects of NG-monomethyl-L-arginine and L-arginine on acetylcholine renal response. Hypertension. 1990 Jun 1;15(6 PART 1):659-663.
Lahera, Vicente ; Salom, Miguel G. ; Fiksen-Olsen, Mary J. ; Raij, Leopoldo ; Romero, J. Carlos. / Effects of NG-monomethyl-L-arginine and L-arginine on acetylcholine renal response. In: Hypertension. 1990 ; Vol. 15, No. 6 PART 1. pp. 659-663.
@article{e79f210ecc0240aba3c13c2e6607733a,
title = "Effects of NG-monomethyl-L-arginine and L-arginine on acetylcholine renal response",
abstract = "Intrarenal infusion of acetylcholine in meclofenamate-treated dogs significantly increased renal blood flow, diuresis, and natriuresis. Intrarenal infusions of either NG-monomethyl-L-arginine (inhibitor of endothelium-derived relaxing factor formation), or L-arginine (precursor of endothelium-derived relaxing factor formation) did not modify basal levels of those parameters. However, the infusion of NG-monomethyl-L-arginine inhibited the acetylcholine-induced increases in renal blood flow and diuresis without affecting natriuresis, which increased significantly. The infusion of L-arginine failed to further enhance hemodynamic and excretory effects elicited by acetylcholine. The concomitant infusion of L-arginine and NG-monomethyl-L-arginine did not change renal blood flow, urine flow, or sodium excretion rate. L-Arginine administration prevented the inhibitory effect of NG-monomethyl-L-arginine on acetylcholine-induced renal vasodilatation and diuresis. Glomerular filtration rate and mean arterial pressure did not change throughout the experiment. The results indicate that the vasodilatory and diuretic responses to intrarenal acetylcholine in meclofenamate-treated dogs are largely dependent on endothelium-derived relaxing factor.",
keywords = "Acetylcholine, Arginine, Endothelium, Hemodynamics, Renal function",
author = "Vicente Lahera and Salom, {Miguel G.} and Fiksen-Olsen, {Mary J.} and Leopoldo Raij and Romero, {J. Carlos}",
year = "1990",
month = "6",
day = "1",
language = "English",
volume = "15",
pages = "659--663",
journal = "Hypertension",
issn = "0194-911X",
publisher = "Lippincott Williams and Wilkins",
number = "6 PART 1",

}

TY - JOUR

T1 - Effects of NG-monomethyl-L-arginine and L-arginine on acetylcholine renal response

AU - Lahera, Vicente

AU - Salom, Miguel G.

AU - Fiksen-Olsen, Mary J.

AU - Raij, Leopoldo

AU - Romero, J. Carlos

PY - 1990/6/1

Y1 - 1990/6/1

N2 - Intrarenal infusion of acetylcholine in meclofenamate-treated dogs significantly increased renal blood flow, diuresis, and natriuresis. Intrarenal infusions of either NG-monomethyl-L-arginine (inhibitor of endothelium-derived relaxing factor formation), or L-arginine (precursor of endothelium-derived relaxing factor formation) did not modify basal levels of those parameters. However, the infusion of NG-monomethyl-L-arginine inhibited the acetylcholine-induced increases in renal blood flow and diuresis without affecting natriuresis, which increased significantly. The infusion of L-arginine failed to further enhance hemodynamic and excretory effects elicited by acetylcholine. The concomitant infusion of L-arginine and NG-monomethyl-L-arginine did not change renal blood flow, urine flow, or sodium excretion rate. L-Arginine administration prevented the inhibitory effect of NG-monomethyl-L-arginine on acetylcholine-induced renal vasodilatation and diuresis. Glomerular filtration rate and mean arterial pressure did not change throughout the experiment. The results indicate that the vasodilatory and diuretic responses to intrarenal acetylcholine in meclofenamate-treated dogs are largely dependent on endothelium-derived relaxing factor.

AB - Intrarenal infusion of acetylcholine in meclofenamate-treated dogs significantly increased renal blood flow, diuresis, and natriuresis. Intrarenal infusions of either NG-monomethyl-L-arginine (inhibitor of endothelium-derived relaxing factor formation), or L-arginine (precursor of endothelium-derived relaxing factor formation) did not modify basal levels of those parameters. However, the infusion of NG-monomethyl-L-arginine inhibited the acetylcholine-induced increases in renal blood flow and diuresis without affecting natriuresis, which increased significantly. The infusion of L-arginine failed to further enhance hemodynamic and excretory effects elicited by acetylcholine. The concomitant infusion of L-arginine and NG-monomethyl-L-arginine did not change renal blood flow, urine flow, or sodium excretion rate. L-Arginine administration prevented the inhibitory effect of NG-monomethyl-L-arginine on acetylcholine-induced renal vasodilatation and diuresis. Glomerular filtration rate and mean arterial pressure did not change throughout the experiment. The results indicate that the vasodilatory and diuretic responses to intrarenal acetylcholine in meclofenamate-treated dogs are largely dependent on endothelium-derived relaxing factor.

KW - Acetylcholine

KW - Arginine

KW - Endothelium

KW - Hemodynamics

KW - Renal function

UR - http://www.scopus.com/inward/record.url?scp=0025437781&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025437781&partnerID=8YFLogxK

M3 - Article

C2 - 2347626

AN - SCOPUS:0025437781

VL - 15

SP - 659

EP - 663

JO - Hypertension

JF - Hypertension

SN - 0194-911X

IS - 6 PART 1

ER -