Effects of neurotensin on caudate nucleus protein phosphorylation

Scott T. Cain, Murray Abramson, Charles Nemeroff

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The tridecapeptide, neurotensin (NT), is heterogenously distributed in the mammalian central nervous system and exhibits many neurotransmitter-like characteristics. However, the molecular mechanisms of NT signal transduction remain obscure. In this report, we demonstrate NT-induced stimulation of specific protein substrate phosphorylation in the rat caudate nucleus. Rat caudate nucleus was dissected, a P2 fraction prepared and proteins phosphorylated in vitro with [32P]ATP for 1 min. Phosphorylated proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and autoradiograms prepared. NT preincubation in the absence of calcium resulted in markedly increased phosphorylation in vitro of proteins with apparent molecular weights of 80,000 and 50,000. These effects were not observed if calcium was present during the NT preincubation period. Both calcium and cAMP enhanced phosphorylation of the 80 kDa protein, but phosphorylation of the 50 kDa protein was responsive only to calcium.

Original languageEnglish
Pages (from-to)55-65
Number of pages11
JournalRegulatory Peptides
Volume39
Issue number1
DOIs
StatePublished - Apr 29 1992
Externally publishedYes

Fingerprint

Neurotensin
Phosphorylation
Caudate Nucleus
Calcium
Proteins
Rats
Signal transduction
Neurology
Electrophoresis
Sodium Dodecyl Sulfate
Neurotransmitter Agents
Polyacrylamide Gel Electrophoresis
Signal Transduction
Central Nervous System
Adenosine Triphosphate
Molecular Weight
Molecular weight
Substrates

Keywords

  • Calcium
  • Caudate nucleus
  • Electrophoresis
  • Neurotensin
  • P fraction
  • Protein phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Effects of neurotensin on caudate nucleus protein phosphorylation. / Cain, Scott T.; Abramson, Murray; Nemeroff, Charles.

In: Regulatory Peptides, Vol. 39, No. 1, 29.04.1992, p. 55-65.

Research output: Contribution to journalArticle

Cain, Scott T. ; Abramson, Murray ; Nemeroff, Charles. / Effects of neurotensin on caudate nucleus protein phosphorylation. In: Regulatory Peptides. 1992 ; Vol. 39, No. 1. pp. 55-65.
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