Effects of neuropeptide Y (NPY) on isolated guinea-pig heart

Claes R Wahlestedt, B. Wohlfart, R. Hakanson

Research output: Contribution to journalArticle

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Abstract

Neuropeptide Y (NPY) is present in nerve fibres throughout the mammalian heart. We have elucidated the effects of NPY on the isolated papillary muscle and heart (Langendorff) from the guinea pig. The paced papillary muscle was studied with regard to duration of the action potential, peak force, maximum rate of force development, time to peak force, and time from peak force to half relaxation; all these parameters were identical whether or not NPY (5 x 10-7 M) was present in the medium. When a stimulation with trains of pulses was superimposed, the paced papillary muscle exhibited enhanced contractions. This increase in contractility was not observed in the presence of the β-adrenoceptor antagonist propranolol (10-6 M) and was thus considered to be adrenergically mediated. The latter (adrenergic) response was markedly attenuated to NPY. Since NPY did not interfere with the response to exogenous noradrenaline (NA) it is suggested that the peptide exerts a pre-junctional inhibitory affect on adrenergic nerve-mediated positive inotropy. Neuropeptide Y did not influence the electrocardiogram from the spontaneously beating heart (Lagendorff), nor did the peptide modify the positive chronotropic effect of exogenously applied NA. In conclusion, the results indicate that NPY is without effect on the heart muscle proper but that the sympathetic terminals of the heart possess pre-junctional receptors for NPY (and/or related peptides) related to suppression of stimulated NA release.

Original languageEnglish
Pages (from-to)459-463
Number of pages5
JournalActa Physiologica Scandinavica
Volume129
Issue number4
StatePublished - Jan 1 1987
Externally publishedYes

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Neuropeptide Y
Guinea Pigs
Papillary Muscles
Norepinephrine
Adrenergic Agents
Peptides
Neuropeptide Y Receptors
Nerve Fibers
Propranolol
Adrenergic Receptors
Action Potentials
Myocardium
Electrocardiography

ASJC Scopus subject areas

  • Physiology

Cite this

Effects of neuropeptide Y (NPY) on isolated guinea-pig heart. / Wahlestedt, Claes R; Wohlfart, B.; Hakanson, R.

In: Acta Physiologica Scandinavica, Vol. 129, No. 4, 01.01.1987, p. 459-463.

Research output: Contribution to journalArticle

Wahlestedt, Claes R ; Wohlfart, B. ; Hakanson, R. / Effects of neuropeptide Y (NPY) on isolated guinea-pig heart. In: Acta Physiologica Scandinavica. 1987 ; Vol. 129, No. 4. pp. 459-463.
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