@article{6160adf5c9694438b9ce1a28e4bdbe88,
title = "Effects of Mitomycin-C and 5-Fluorouracil on Ocular Adnexal Sebaceous Carcinoma Cells",
abstract = "PURPOSE: To investigate the effects of mitomycin-C (MMC) and 5-fluorouracil (5-FU) on the viability, proliferation, and migratory capacity of cultured ocular adnexal sebaceous carcinoma (SC) cells. DESIGN: Laboratory investigation. METHODS: Human SC cell lines (Bascom Palmer 50 and 52 [BP50 and BP52]) and human limbal stem cells (LSCs) were treated with various concentrations of MMC and 5-FU. Cytotoxicity was assessed with the tetrazolium MTT colorimetric viability assay on normal corneal vs tumor cells. Growth curves and scratch assays were performed to characterize the effects of these chemotherapeutic agents on SC proliferation and migration, respectively. RESULTS: MMC decreased BP52 cell viability in a dose-dependent manner with a half-maximal effective dose (EC50) of 11.8 μM after 72 hours. SC viability decreased >50% at 80 mM 5-FU after 72 hours. MMC reduced LSC viability in a dose-dependent manner with an EC50 value of 3.24 μM, and 5-FU decreased LSC viability >50% at 160 μM. MMC decreased SC cell proliferation and migration in a dose-dependent manner. 5-FU displayed antiproliferative effects but did not affect cell migration at concentrations below 1000 μM. CONCLUSIONS: Our in vitro data corroborate clinical observations that MMC is efficacious for treating ocular adnexal SC, albeit at the expense of LSC viability. Our findings also demonstrate that topical 5-FU exhibits antiproliferative effects that supersede its cancer-killing and antimigratory effects on cultured SC cells.",
author = "Gallo, {Ryan A.} and Lang, {Steven H.} and Angela Gomez and Sabater, {Alfonso L.} and Tse, {David T.} and Daniel Pelaez and Rong, {Andrew J.}",
note = "Funding Information: Funding/Support: This work was supported in part by the Patrick P. Lee Foundation (Williamsville, New York) and the Dr. Nasser Al-Rashid Orbital Research Endowment (Miami, Florida). Bascom Palmer Eye Institute is supported by NIH Center Core Grant P30EY014801 and a Research to Prevent Blindness Unrestricted Grant (New York, New York). R.G. was supported in part by NIH Medical Scientist Training Program T32 GM112601. Financial Disclosures: The authors indicate no financial support or conflicts of interest. All authors attest that they meet the current ICMJE criteria for authorship. Acknowledgements: The authors thank the Florida Lions Eye Bank (Miami, Florida, USA) for assisting with cornea tissue procurement. Funding Information: Funding/Support: This work was supported in part by the Patrick P. Lee Foundation (Williamsville, New York) and the Dr. Nasser Al-Rashid Orbital Research Endowment (Miami, Florida). Bascom Palmer Eye Institute is supported by NIH Center Core Grant P30EY014801 and a Research to Prevent Blindness Unrestricted Grant (New York, New York). R.G. was supported in part by NIH Medical Scientist Training Program T32 GM112601. Financial Disclosures: The authors indicate no financial support or conflicts of interest. All authors attest that they meet the current ICMJE criteria for authorship. Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",
year = "2022",
month = aug,
doi = "10.1016/j.ajo.2021.12.016",
language = "English (US)",
volume = "240",
pages = "14--22",
journal = "American Journal of Ophthalmology",
issn = "0002-9394",
publisher = "Elsevier USA",
}
