Abstract
Traumatic brain injury (TBI) produces transient increases in constitutive nitric oxide synthase (cNOS) activity and prolonged behavioral abnormalities. This study investigated the effects of nitro-L-arginine- methyl ester (L-NAME) and 3-bromo-7-nitroindazole (7-NI) treatment on cNOS catalytic activity and sensorimotor behavioral outcome after TBI. Rats underwent moderate (1.8-2.2 atm) parasagittal fluid percussion brain injury (FPI). At 5 min after FPI, cNOS activity was significantly increased within the damaged cerebral cortex of vehicle-treated rats compared to the noninjured contralateral cortex (206.7 ± 150.5 % of contralateral, p < 0.01). Pretreatment with L-NAME and 7-NI significantly reduced injury- induced cNOS activation (47.7 ± 42.6%, p < 0.05, and 96.16 ± 12.76, p < 0.05, respectively). Pretreatment with L-NAME and 7-NI also inhibited cNOS activity within the contralateral noninjured cerebral cortex compared to vehicle-treated rats (L-NAME 43.7 ± 12.47%, p < 0.05; 7-NI 36.8 ± 7.47%, p < 0.05). Furthermore, pretreatment with 7-NI, but not L-NAME, significantly reduced forelimb placing sensorimotor deficits (3.14 ± 1.07, p < 0.05) at 1 day after TBI compared to vehicle-treated rats (5.38 ± 0.42). These data indicate that inhibition of injury-induced elevations in neuronal NOS activity has a beneficial effect on neurological outcome after parasagittal FPI brain injury.
| Original language | English |
|---|---|
| Pages (from-to) | 203-212 |
| Number of pages | 10 |
| Journal | Journal of Neurotrauma |
| Volume | 16 |
| Issue number | 3 |
| State | Published - Mar 1 1999 |
Fingerprint
Keywords
- 3-bromo-7-nitro indazole
- Behavior
- Fluid percussion brain injury
- Nitric oxide synthase
- Nitro-L-arginine-methyl ester
- Rat
ASJC Scopus subject areas
- Clinical Neurology
- Neuroscience(all)
Cite this
Effects of L-NAME and 7-NI on NOS catalytic activity and behavioral outcome after traumatic brain injury in the rat. / Wada, Kojiro; Chatzipanteli, Katina; Busto, Raul; Dalton Dietrich, W.
In: Journal of Neurotrauma, Vol. 16, No. 3, 01.03.1999, p. 203-212.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of L-NAME and 7-NI on NOS catalytic activity and behavioral outcome after traumatic brain injury in the rat
AU - Wada, Kojiro
AU - Chatzipanteli, Katina
AU - Busto, Raul
AU - Dalton Dietrich, W.
PY - 1999/3/1
Y1 - 1999/3/1
N2 - Traumatic brain injury (TBI) produces transient increases in constitutive nitric oxide synthase (cNOS) activity and prolonged behavioral abnormalities. This study investigated the effects of nitro-L-arginine- methyl ester (L-NAME) and 3-bromo-7-nitroindazole (7-NI) treatment on cNOS catalytic activity and sensorimotor behavioral outcome after TBI. Rats underwent moderate (1.8-2.2 atm) parasagittal fluid percussion brain injury (FPI). At 5 min after FPI, cNOS activity was significantly increased within the damaged cerebral cortex of vehicle-treated rats compared to the noninjured contralateral cortex (206.7 ± 150.5 % of contralateral, p < 0.01). Pretreatment with L-NAME and 7-NI significantly reduced injury- induced cNOS activation (47.7 ± 42.6%, p < 0.05, and 96.16 ± 12.76, p < 0.05, respectively). Pretreatment with L-NAME and 7-NI also inhibited cNOS activity within the contralateral noninjured cerebral cortex compared to vehicle-treated rats (L-NAME 43.7 ± 12.47%, p < 0.05; 7-NI 36.8 ± 7.47%, p < 0.05). Furthermore, pretreatment with 7-NI, but not L-NAME, significantly reduced forelimb placing sensorimotor deficits (3.14 ± 1.07, p < 0.05) at 1 day after TBI compared to vehicle-treated rats (5.38 ± 0.42). These data indicate that inhibition of injury-induced elevations in neuronal NOS activity has a beneficial effect on neurological outcome after parasagittal FPI brain injury.
AB - Traumatic brain injury (TBI) produces transient increases in constitutive nitric oxide synthase (cNOS) activity and prolonged behavioral abnormalities. This study investigated the effects of nitro-L-arginine- methyl ester (L-NAME) and 3-bromo-7-nitroindazole (7-NI) treatment on cNOS catalytic activity and sensorimotor behavioral outcome after TBI. Rats underwent moderate (1.8-2.2 atm) parasagittal fluid percussion brain injury (FPI). At 5 min after FPI, cNOS activity was significantly increased within the damaged cerebral cortex of vehicle-treated rats compared to the noninjured contralateral cortex (206.7 ± 150.5 % of contralateral, p < 0.01). Pretreatment with L-NAME and 7-NI significantly reduced injury- induced cNOS activation (47.7 ± 42.6%, p < 0.05, and 96.16 ± 12.76, p < 0.05, respectively). Pretreatment with L-NAME and 7-NI also inhibited cNOS activity within the contralateral noninjured cerebral cortex compared to vehicle-treated rats (L-NAME 43.7 ± 12.47%, p < 0.05; 7-NI 36.8 ± 7.47%, p < 0.05). Furthermore, pretreatment with 7-NI, but not L-NAME, significantly reduced forelimb placing sensorimotor deficits (3.14 ± 1.07, p < 0.05) at 1 day after TBI compared to vehicle-treated rats (5.38 ± 0.42). These data indicate that inhibition of injury-induced elevations in neuronal NOS activity has a beneficial effect on neurological outcome after parasagittal FPI brain injury.
KW - 3-bromo-7-nitro indazole
KW - Behavior
KW - Fluid percussion brain injury
KW - Nitric oxide synthase
KW - Nitro-L-arginine-methyl ester
KW - Rat
UR - http://www.scopus.com/inward/record.url?scp=0033035867&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033035867&partnerID=8YFLogxK
M3 - Article
VL - 16
SP - 203
EP - 212
JO - Journal of Neurotrauma
JF - Journal of Neurotrauma
SN - 0897-7151
IS - 3
ER -