Abstract
Harman and norharman, two β-carboline derivatives known to exist in certain foods and to be formed during pyrolysis of tobacco and meat, were tested for mutagenic activity in the presence of benzo[a]pyrene, mouse liver enzymes, and Salmonella typhimurium TA98 in vitro. Both harman and norharman inhibit benzo[a]pyrene mutagenicity, benzo[a]pyrene metabolism (as measured by aryl hydrocarbon hydroxylase activity), and the binding of all benzo[a]pyrene metabolites to DNA in vitro. Moreover, harman and norharman are quite toxic to cultures of hepatoma-derived H-4-II-E and Hepa-1 established cell lines and therefore were found to be very weak inducers of aryl hydrocarbon hydroxylase activity.
| Original language | English |
|---|---|
| Pages (from-to) | 1167-1175 |
| Number of pages | 9 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 79 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 21 1977 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Effects of harman and norharman on the mutagenicity and binding to DNA of benzo[a]pyrene metabolites in vitro and on aryl hydrocarbon hydroxylase induction in cell culture. / Levitt, Roy C; Legraverend, Catherine; Nebert, Daniel W.; Pelkonen, Olavi.
In: Biochemical and Biophysical Research Communications, Vol. 79, No. 4, 21.12.1977, p. 1167-1175.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of harman and norharman on the mutagenicity and binding to DNA of benzo[a]pyrene metabolites in vitro and on aryl hydrocarbon hydroxylase induction in cell culture
AU - Levitt, Roy C
AU - Legraverend, Catherine
AU - Nebert, Daniel W.
AU - Pelkonen, Olavi
PY - 1977/12/21
Y1 - 1977/12/21
N2 - Harman and norharman, two β-carboline derivatives known to exist in certain foods and to be formed during pyrolysis of tobacco and meat, were tested for mutagenic activity in the presence of benzo[a]pyrene, mouse liver enzymes, and Salmonella typhimurium TA98 in vitro. Both harman and norharman inhibit benzo[a]pyrene mutagenicity, benzo[a]pyrene metabolism (as measured by aryl hydrocarbon hydroxylase activity), and the binding of all benzo[a]pyrene metabolites to DNA in vitro. Moreover, harman and norharman are quite toxic to cultures of hepatoma-derived H-4-II-E and Hepa-1 established cell lines and therefore were found to be very weak inducers of aryl hydrocarbon hydroxylase activity.
AB - Harman and norharman, two β-carboline derivatives known to exist in certain foods and to be formed during pyrolysis of tobacco and meat, were tested for mutagenic activity in the presence of benzo[a]pyrene, mouse liver enzymes, and Salmonella typhimurium TA98 in vitro. Both harman and norharman inhibit benzo[a]pyrene mutagenicity, benzo[a]pyrene metabolism (as measured by aryl hydrocarbon hydroxylase activity), and the binding of all benzo[a]pyrene metabolites to DNA in vitro. Moreover, harman and norharman are quite toxic to cultures of hepatoma-derived H-4-II-E and Hepa-1 established cell lines and therefore were found to be very weak inducers of aryl hydrocarbon hydroxylase activity.
UR - http://www.scopus.com/inward/record.url?scp=0017566629&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017566629&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(77)91129-9
DO - 10.1016/0006-291X(77)91129-9
M3 - Article
VL - 79
SP - 1167
EP - 1175
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -