Effects of harman and norharman on the mutagenicity and binding to DNA of benzo[a]pyrene metabolites in vitro and on aryl hydrocarbon hydroxylase induction in cell culture

Roy C Levitt, Catherine Legraverend, Daniel W. Nebert, Olavi Pelkonen

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Harman and norharman, two β-carboline derivatives known to exist in certain foods and to be formed during pyrolysis of tobacco and meat, were tested for mutagenic activity in the presence of benzo[a]pyrene, mouse liver enzymes, and Salmonella typhimurium TA98 in vitro. Both harman and norharman inhibit benzo[a]pyrene mutagenicity, benzo[a]pyrene metabolism (as measured by aryl hydrocarbon hydroxylase activity), and the binding of all benzo[a]pyrene metabolites to DNA in vitro. Moreover, harman and norharman are quite toxic to cultures of hepatoma-derived H-4-II-E and Hepa-1 established cell lines and therefore were found to be very weak inducers of aryl hydrocarbon hydroxylase activity.

Original languageEnglish
Pages (from-to)1167-1175
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume79
Issue number4
DOIs
StatePublished - Dec 21 1977
Externally publishedYes

Fingerprint

norharman
Aryl Hydrocarbon Hydroxylases
Benzo(a)pyrene
Metabolites
Cell culture
Cell Culture Techniques
DNA
Carbolines
Salmonella
Tobacco
Meats
Poisons
Salmonella typhimurium
Metabolism
Liver
Meat
Hepatocellular Carcinoma
Pyrolysis
Cells
Derivatives

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effects of harman and norharman on the mutagenicity and binding to DNA of benzo[a]pyrene metabolites in vitro and on aryl hydrocarbon hydroxylase induction in cell culture. / Levitt, Roy C; Legraverend, Catherine; Nebert, Daniel W.; Pelkonen, Olavi.

In: Biochemical and Biophysical Research Communications, Vol. 79, No. 4, 21.12.1977, p. 1167-1175.

Research output: Contribution to journalArticle

@article{6f074c4c09e843cfa6b540ab3078772a,
title = "Effects of harman and norharman on the mutagenicity and binding to DNA of benzo[a]pyrene metabolites in vitro and on aryl hydrocarbon hydroxylase induction in cell culture",
abstract = "Harman and norharman, two β-carboline derivatives known to exist in certain foods and to be formed during pyrolysis of tobacco and meat, were tested for mutagenic activity in the presence of benzo[a]pyrene, mouse liver enzymes, and Salmonella typhimurium TA98 in vitro. Both harman and norharman inhibit benzo[a]pyrene mutagenicity, benzo[a]pyrene metabolism (as measured by aryl hydrocarbon hydroxylase activity), and the binding of all benzo[a]pyrene metabolites to DNA in vitro. Moreover, harman and norharman are quite toxic to cultures of hepatoma-derived H-4-II-E and Hepa-1 established cell lines and therefore were found to be very weak inducers of aryl hydrocarbon hydroxylase activity.",
author = "Levitt, {Roy C} and Catherine Legraverend and Nebert, {Daniel W.} and Olavi Pelkonen",
year = "1977",
month = "12",
day = "21",
doi = "10.1016/0006-291X(77)91129-9",
language = "English",
volume = "79",
pages = "1167--1175",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Effects of harman and norharman on the mutagenicity and binding to DNA of benzo[a]pyrene metabolites in vitro and on aryl hydrocarbon hydroxylase induction in cell culture

AU - Levitt, Roy C

AU - Legraverend, Catherine

AU - Nebert, Daniel W.

AU - Pelkonen, Olavi

PY - 1977/12/21

Y1 - 1977/12/21

N2 - Harman and norharman, two β-carboline derivatives known to exist in certain foods and to be formed during pyrolysis of tobacco and meat, were tested for mutagenic activity in the presence of benzo[a]pyrene, mouse liver enzymes, and Salmonella typhimurium TA98 in vitro. Both harman and norharman inhibit benzo[a]pyrene mutagenicity, benzo[a]pyrene metabolism (as measured by aryl hydrocarbon hydroxylase activity), and the binding of all benzo[a]pyrene metabolites to DNA in vitro. Moreover, harman and norharman are quite toxic to cultures of hepatoma-derived H-4-II-E and Hepa-1 established cell lines and therefore were found to be very weak inducers of aryl hydrocarbon hydroxylase activity.

AB - Harman and norharman, two β-carboline derivatives known to exist in certain foods and to be formed during pyrolysis of tobacco and meat, were tested for mutagenic activity in the presence of benzo[a]pyrene, mouse liver enzymes, and Salmonella typhimurium TA98 in vitro. Both harman and norharman inhibit benzo[a]pyrene mutagenicity, benzo[a]pyrene metabolism (as measured by aryl hydrocarbon hydroxylase activity), and the binding of all benzo[a]pyrene metabolites to DNA in vitro. Moreover, harman and norharman are quite toxic to cultures of hepatoma-derived H-4-II-E and Hepa-1 established cell lines and therefore were found to be very weak inducers of aryl hydrocarbon hydroxylase activity.

UR - http://www.scopus.com/inward/record.url?scp=0017566629&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017566629&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(77)91129-9

DO - 10.1016/0006-291X(77)91129-9

M3 - Article

VL - 79

SP - 1167

EP - 1175

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -