Effects of growth hormone-releasing hormone agonistic analog MR-409 on insulin-secreting cells under cyclopiazonic acid-induced endoplasmic reticulum stress

Karina Rodrigues-dos-Santos, Gabriela M. Soares, Dimitrius S.P.S.F. Guimarães, Thiago R. Araújo, Jean F. Vettorazzi, Lucas Zangerolamo, Emilio Marconato-Júnior, Renzhi Cai, Wei Sha, Andrew V. Schally, Antônio C. Boschero, Helena C.L. Barbosa

Research output: Contribution to journalArticlepeer-review

Abstract

The endoplasmic reticulum (ER) stress is one of the mechanisms related to decreased insulin secretion and beta cell death, contributing to the progress of type 2 diabetes mellitus (T2D). Thus, investigating agents that can influence this process would help prevent the development of T2D. Recently, the growth-hormone-releasing hormone (GHRH) action has been demonstrated in INS-1E cells, in which it increases cell proliferation and insulin secretion. As the effects of GHRH and its agonists have not been fully elucidated in the beta cell, we proposed to investigate them by evaluating the role of the GHRH agonist, MR-409, in cells under ER stress. Our results show that the agonist was unable to ameliorate or prevent ER stress. However, cells exposed to the agonist showed less oxidative stress and greater survival even under ER stress. The mechanisms by which GHRH agonist, MR-409, leads to these outcomes require further investigation.

Original languageEnglish (US)
Article number111379
JournalMolecular and Cellular Endocrinology
Volume535
DOIs
StatePublished - Sep 15 2021

Keywords

  • Beta cell
  • Endoplasmic reticulum stress
  • GHRH agonists
  • Oxidative stress
  • Type 2 diabetes
  • Unfolded protein response

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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