Abstract
The effects of various Ca2+ concentrations and glucose deprivation on cell proliferation, insulin secretion and contents of insulin, calcium and magnesium were studied in a clonal cell line (RINm5F) established from a transplantable rat islet tumor. The RINm5F cells were equally effective in proliferating and releasing insulin at Ca2+ concentrations ranging from 0.16 to 4.4 mM. In contradistinction to normal β-cells, the amounts of calcium in the RINm5F cells remained relatively constant after lowering the extracellular concentration of Ca2+ and inhibiting the entrance of the ion with D-600. However, culture at 4.4 mM Ca2+ resulted in substantial incorporation of cellular calcium and a simultaneous reduction of the insulin content. Cells deprived of glucose had a significantly lower dry weight, were less able to exclude trypan blue and contained and released less insulin. Since protein biosynthesis is strictly dependent upon an adequate intracellular concentration of Mg2+, these long term effects of glucose removal might be related to the concomitant decrease in the cellular content of magnesium.
Original language | English (US) |
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Pages (from-to) | 77-84 |
Number of pages | 8 |
Journal | Biomedica Biochimica Acta |
Volume | 44 |
Issue number | 1 |
State | Published - Jan 1 1985 |
ASJC Scopus subject areas
- Biochemistry