Abstract
The effects of various Ca2+ concentrations and glucose deprivation on cell proliferation, insulin secretion and contents of insulin, calcium and magnesium were studied in a clonal cell line (RINm5F) established from a transplantable rat islet tumor. The RINm5F cells were equally effective in proliferating and releasing insulin at Ca2+ concentrations ranging from 0.16 to 4.4 mM. In contradistinction to normal β-cells, the amounts of calcium in the RINm5F cells remained relatively constant after lowering the extracellular concentration of Ca2+ and inhibiting the entrance of the ion with D-600. However, culture at 4.4 mM Ca2+ resulted in substantial incorporation of cellular calcium and a simultaneous reduction of the insulin content. Cells deprived of glucose had a significantly lower dry weight, were less able to exclude trypan blue and contained and released less insulin. Since protein biosynthesis is strictly dependent upon an adequate intracellular concentration of Mg2+, these long term effects of glucose removal might be related to the concomitant decrease in the cellular content of magnesium.
| Original language | English |
|---|---|
| Pages (from-to) | 77-84 |
| Number of pages | 8 |
| Journal | Biomedica Biochimica Acta |
| Volume | 44 |
| Issue number | 1 |
| State | Published - Jan 1 1985 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Effects of glucose deprivation and altered Ca2+ concentrations on clonal insulin-producing cells (RINm5F). / Hoftiezer, V.; Berggren, P. O.; Hellman, B.
In: Biomedica Biochimica Acta, Vol. 44, No. 1, 01.01.1985, p. 77-84.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of glucose deprivation and altered Ca2+ concentrations on clonal insulin-producing cells (RINm5F)
AU - Hoftiezer, V.
AU - Berggren, P. O.
AU - Hellman, B.
PY - 1985/1/1
Y1 - 1985/1/1
N2 - The effects of various Ca2+ concentrations and glucose deprivation on cell proliferation, insulin secretion and contents of insulin, calcium and magnesium were studied in a clonal cell line (RINm5F) established from a transplantable rat islet tumor. The RINm5F cells were equally effective in proliferating and releasing insulin at Ca2+ concentrations ranging from 0.16 to 4.4 mM. In contradistinction to normal β-cells, the amounts of calcium in the RINm5F cells remained relatively constant after lowering the extracellular concentration of Ca2+ and inhibiting the entrance of the ion with D-600. However, culture at 4.4 mM Ca2+ resulted in substantial incorporation of cellular calcium and a simultaneous reduction of the insulin content. Cells deprived of glucose had a significantly lower dry weight, were less able to exclude trypan blue and contained and released less insulin. Since protein biosynthesis is strictly dependent upon an adequate intracellular concentration of Mg2+, these long term effects of glucose removal might be related to the concomitant decrease in the cellular content of magnesium.
AB - The effects of various Ca2+ concentrations and glucose deprivation on cell proliferation, insulin secretion and contents of insulin, calcium and magnesium were studied in a clonal cell line (RINm5F) established from a transplantable rat islet tumor. The RINm5F cells were equally effective in proliferating and releasing insulin at Ca2+ concentrations ranging from 0.16 to 4.4 mM. In contradistinction to normal β-cells, the amounts of calcium in the RINm5F cells remained relatively constant after lowering the extracellular concentration of Ca2+ and inhibiting the entrance of the ion with D-600. However, culture at 4.4 mM Ca2+ resulted in substantial incorporation of cellular calcium and a simultaneous reduction of the insulin content. Cells deprived of glucose had a significantly lower dry weight, were less able to exclude trypan blue and contained and released less insulin. Since protein biosynthesis is strictly dependent upon an adequate intracellular concentration of Mg2+, these long term effects of glucose removal might be related to the concomitant decrease in the cellular content of magnesium.
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M3 - Article
VL - 44
SP - 77
EP - 84
JO - Biomedica Biochimica Acta
JF - Biomedica Biochimica Acta
SN - 0232-766X
IS - 1
ER -