Effects of general anaesthetic procedures on mitochondrial function of human skeletal muscle

Ò Miró, Antonio Barrientos, J. R. Alonso, J. Casademont, D. Jarreta, Á Urbano-Márquez, F. Cardellach

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Background: General anaesthetics inhibit mitochondrial function in animal models. However, very few studies have been performed in humans, and the results have not been conclusive. Methods: We prospectively studied the oxygen consumption and the individual enzyme activity of each complex of the mitochondrial respiratory chain of skeletal muscle mitochondria in 54 healthy individuals who underwent general anaesthesia for orthopaedic surgery. The control group (n = 54) was made up of individuals submitted to the same orthopaedic procedure under regional anaesthesia (n = 31), and patients who underwent muscle biopsies for diagnostic purposes by local anaesthesia (n = 23). Results: We found a significant decrease in the oxidation of glutamate (-36%), succinate (-25%) and ascorbate (-29%) in the general anaesthetic group compared with the controls (P < 0.001 for all substrates). The level of such inhibition was similar for volatile anaesthetics with strong (halothane) or weak (isoflurane) negative inotropic effect. By contrast, the enzymatic activity of all individual complexes and the coupling of oxidative phosphorylation did not differ between the two groups. Conclusion: We conclude that during general anaesthetic procedures there is an extensive inhibition of substrate oxidation in human muscle mitochondria, and that it is not caused by a direct effect on complexes of the mitochondrial respiratory chain or through uncoupling oxidative phosphorylation.

Original languageEnglish
Pages (from-to)35-41
Number of pages7
JournalEuropean Journal of Clinical Pharmacology
Volume55
Issue number1
DOIs
StatePublished - Apr 6 1999

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General Anesthetics
Muscle Mitochondrion
Skeletal Muscle
Oxidative Phosphorylation
Electron Transport
Orthopedic Procedures
Conduction Anesthesia
Isoflurane
Succinic Acid
Halothane
Local Anesthesia
Oxygen Consumption
General Anesthesia
Orthopedics
Anesthetics
Glutamic Acid
Animal Models
Biopsy
Muscles
Control Groups

Keywords

  • Electron transport chain
  • General anaesthetics
  • Halothane Isoflurane
  • Mitochondria
  • Respiratory chain

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Effects of general anaesthetic procedures on mitochondrial function of human skeletal muscle. / Miró, Ò; Barrientos, Antonio; Alonso, J. R.; Casademont, J.; Jarreta, D.; Urbano-Márquez, Á; Cardellach, F.

In: European Journal of Clinical Pharmacology, Vol. 55, No. 1, 06.04.1999, p. 35-41.

Research output: Contribution to journalArticle

Miró, Ò, Barrientos, A, Alonso, JR, Casademont, J, Jarreta, D, Urbano-Márquez, Á & Cardellach, F 1999, 'Effects of general anaesthetic procedures on mitochondrial function of human skeletal muscle', European Journal of Clinical Pharmacology, vol. 55, no. 1, pp. 35-41. https://doi.org/10.1007/s002280050589
Miró, Ò ; Barrientos, Antonio ; Alonso, J. R. ; Casademont, J. ; Jarreta, D. ; Urbano-Márquez, Á ; Cardellach, F. / Effects of general anaesthetic procedures on mitochondrial function of human skeletal muscle. In: European Journal of Clinical Pharmacology. 1999 ; Vol. 55, No. 1. pp. 35-41.
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