Abstract
Objective: To evaluate the effects of four weeks of fish oil supplementation on apolipoprotein B100 production and lipoprotein metabolism in normolipidaemic males. Design and subjects: Very low density lipoprotein (VLDL) apolipoprotein B100 (apoB100) kinetics in ten healthy, white males, aged 22-43 y (mean 32 y) were investigated using a 13C-leucine technique and gas chromatography-mass spectrometry before and after fish oil supplementation. Intervention: All subjects received 10 g (1.8 g EPA, 1.2 g DHA)/d of fish oil concentrate for four weeks. Results: Fish oil supplementation resulted in a decrease of total plasma VLDL (mean ± s.d. 1.11 ± 0.41 vs 0.87 ± 0.28 mmol/l, P < 0.05) and triacylglycerol concentrations (0.74 ± 0.27 vs 0.48 ± 0.21 mmol/l, P < 0.01). VLDL apoB100 pool size was decreased without alteration of the fractional synthetic rate but a significant decrease of apoB100 production (2.23 ± 0.90 vs 1.54 ± 0.52 mg/dl/h, P < 0.02). Following fish oil supplementation plasma concentrations of glucose and insulin as well as lipoprotein and hepatic lipase activities were unchanged. Fasting plasma concentrations of non-esterified fatty acid (NEFA) were decreased (0.45 ± 0.12 vs 0.33 ± 0.10 mmol/l, P < 0.05). Conclusions: Dietary supplementation with fish oil in healthy males results in decreased VLDL-triacylglycerol concentrations through a decrease in VLDL particle synthesis. The decrease in NEFA substrate supply also contributes.
| Original language | English |
|---|---|
| Pages (from-to) | 104-109 |
| Number of pages | 6 |
| Journal | European Journal of Clinical Nutrition |
| Volume | 52 |
| Issue number | 2 |
| State | Published - Feb 1 1998 |
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Keywords
- apoB100
- Fish oil
- Lipoprotein
- Mass spectrometry
- n-3 fatty acids
- Stable isotopes
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Food Science
Cite this
Effects of fish oil supplementation on apolipoprotein B100 production and lipoprotein metabolism in normolipidaemic males. / Bordin, P.; Bodamer, O. A F; Venkatesan, S.; Gray, R. M.; Bannister, P. A.; Halliday, D.
In: European Journal of Clinical Nutrition, Vol. 52, No. 2, 01.02.1998, p. 104-109.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of fish oil supplementation on apolipoprotein B100 production and lipoprotein metabolism in normolipidaemic males
AU - Bordin, P.
AU - Bodamer, O. A F
AU - Venkatesan, S.
AU - Gray, R. M.
AU - Bannister, P. A.
AU - Halliday, D.
PY - 1998/2/1
Y1 - 1998/2/1
N2 - Objective: To evaluate the effects of four weeks of fish oil supplementation on apolipoprotein B100 production and lipoprotein metabolism in normolipidaemic males. Design and subjects: Very low density lipoprotein (VLDL) apolipoprotein B100 (apoB100) kinetics in ten healthy, white males, aged 22-43 y (mean 32 y) were investigated using a 13C-leucine technique and gas chromatography-mass spectrometry before and after fish oil supplementation. Intervention: All subjects received 10 g (1.8 g EPA, 1.2 g DHA)/d of fish oil concentrate for four weeks. Results: Fish oil supplementation resulted in a decrease of total plasma VLDL (mean ± s.d. 1.11 ± 0.41 vs 0.87 ± 0.28 mmol/l, P < 0.05) and triacylglycerol concentrations (0.74 ± 0.27 vs 0.48 ± 0.21 mmol/l, P < 0.01). VLDL apoB100 pool size was decreased without alteration of the fractional synthetic rate but a significant decrease of apoB100 production (2.23 ± 0.90 vs 1.54 ± 0.52 mg/dl/h, P < 0.02). Following fish oil supplementation plasma concentrations of glucose and insulin as well as lipoprotein and hepatic lipase activities were unchanged. Fasting plasma concentrations of non-esterified fatty acid (NEFA) were decreased (0.45 ± 0.12 vs 0.33 ± 0.10 mmol/l, P < 0.05). Conclusions: Dietary supplementation with fish oil in healthy males results in decreased VLDL-triacylglycerol concentrations through a decrease in VLDL particle synthesis. The decrease in NEFA substrate supply also contributes.
AB - Objective: To evaluate the effects of four weeks of fish oil supplementation on apolipoprotein B100 production and lipoprotein metabolism in normolipidaemic males. Design and subjects: Very low density lipoprotein (VLDL) apolipoprotein B100 (apoB100) kinetics in ten healthy, white males, aged 22-43 y (mean 32 y) were investigated using a 13C-leucine technique and gas chromatography-mass spectrometry before and after fish oil supplementation. Intervention: All subjects received 10 g (1.8 g EPA, 1.2 g DHA)/d of fish oil concentrate for four weeks. Results: Fish oil supplementation resulted in a decrease of total plasma VLDL (mean ± s.d. 1.11 ± 0.41 vs 0.87 ± 0.28 mmol/l, P < 0.05) and triacylglycerol concentrations (0.74 ± 0.27 vs 0.48 ± 0.21 mmol/l, P < 0.01). VLDL apoB100 pool size was decreased without alteration of the fractional synthetic rate but a significant decrease of apoB100 production (2.23 ± 0.90 vs 1.54 ± 0.52 mg/dl/h, P < 0.02). Following fish oil supplementation plasma concentrations of glucose and insulin as well as lipoprotein and hepatic lipase activities were unchanged. Fasting plasma concentrations of non-esterified fatty acid (NEFA) were decreased (0.45 ± 0.12 vs 0.33 ± 0.10 mmol/l, P < 0.05). Conclusions: Dietary supplementation with fish oil in healthy males results in decreased VLDL-triacylglycerol concentrations through a decrease in VLDL particle synthesis. The decrease in NEFA substrate supply also contributes.
KW - apoB100
KW - Fish oil
KW - Lipoprotein
KW - Mass spectrometry
KW - n-3 fatty acids
KW - Stable isotopes
UR - http://www.scopus.com/inward/record.url?scp=0031931002&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031931002&partnerID=8YFLogxK
M3 - Article
C2 - 9505154
AN - SCOPUS:0031931002
VL - 52
SP - 104
EP - 109
JO - European Journal of Clinical Nutrition
JF - European Journal of Clinical Nutrition
SN - 0954-3007
IS - 2
ER -