Effects of fibrinolytic inhibitors on chondrogenesis of bone-marrow derived mesenchymal stem cells in fibrin gels

C. Y.C. Huang, H. S. Cheung, M. A. Deitzer

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The objective of this study was to examine the effect of two fibrinolytic inhibitors, aprotinin and aminohexanoic acid, on chondrogenesis of rabbit bone marrow mesenchymal stem cells (BM-MSCs). Rabbit BM-MSCs were obtained from the tibias and femurs of New Zealand White rabbits. Cell-fibrin constructs were made by mixing a cell-fibrinogen (107 cells/ml; 40 mg/ml fibrinogen) solution with a thrombin (5 IU/ml) solution and then divided into four groups: aprotinin control, aprotinin + transforming growth factor β (TGF-β), aminohexanoic acid control, and aminohexanoic acid + TGF-β. Each of these groups was further treated with three different concentrations of inhibitors and the TGF-β groups were treated with 10 ng/ml of TGF-β1. The chondrogenic gene expressions, DNA content, and glycosaminoglycan content of samples were analyzed after 14 days of culture. The aprotinin groups exhibited significantly higher levels of aggrecan gene expression and glycosaminoglycan content than the aminohexanoic acid groups. However, inhibitor neither influenced gene expression of type II collagen nor proliferation (i.e., DNA content) of BM-MSCs. These findings suggest that fibrinolytic inhibitors used to control degradation of fibrin clot may influence TGF-β-induced chondrogenesis of BM-MSCs.

Original languageEnglish (US)
Pages (from-to)5-11
Number of pages7
JournalBiomechanics and Modeling in Mechanobiology
Volume6
Issue number1-2
DOIs
StatePublished - Jan 1 2007

ASJC Scopus subject areas

  • Biotechnology
  • Modeling and Simulation
  • Mechanical Engineering

Fingerprint Dive into the research topics of 'Effects of fibrinolytic inhibitors on chondrogenesis of bone-marrow derived mesenchymal stem cells in fibrin gels'. Together they form a unique fingerprint.

  • Cite this