Effects of fecal microbial transplantation on microbiome and immunity in simian immunodeficiency virus-infected macaques

Tiffany Hensley-McBain, Alexander S. Zevin, Jennifer Manuzak, Elise Smith, Jillian Gile, Charlene Miller, Brian Agricola, Michael Katze, Keith Reeves, Colleen S. Kraft, Stanley Langevin, Nichole R. Klatta

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

An altered intestinal microbiome during chronic human immunodeficiency virus (HIV) infection is associated with mucosal dysfunction, inflammation, and disease progression. We performed a preclinical evaluation of the safety and efficacy of fecal microbiota transplantation (FMT) as a potential therapeutic in HIV-infected individuals. Antiretroviral-treated, chronically simian immunodeficiency virus (SIV)-infected rhesus macaques received antibiotics followed by FMT. The greatest microbiota shift was observed after antibiotic treatment. The bacterial community composition at 2 weeks post-FMT resembled the pre- FMT community structure, although differences in the abundances of minor bacterial populations remained. Immunologically, we observed significant increases in the number of peripheral Th17 and Th22 cells and reduced CD4+ T cell activation in gastrointestinal tissues post-FMT. Importantly, the transplant was well tolerated with no negative clinical side effects. Although this pilot study did not control for the differential contributions of antibiotic treatment and FMT to the observed results, the data suggest that FMT may have beneficial effects that should be further evaluated in larger studies.

Original languageEnglish (US)
Pages (from-to)4981-4989
Number of pages9
JournalJournal of Virology
Volume90
Issue number10
DOIs
StatePublished - May 1 2016
Externally publishedYes

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Simian immunodeficiency virus
Simian Immunodeficiency Virus
Microbiota
Macaca
Immunity
immunity
Anti-Bacterial Agents
antibiotics
HIV
Th17 Cells
Virus Diseases
Fecal Microbiota Transplantation
microbiome
Macaca mulatta
Disease Progression
Human immunodeficiency virus
HIV infections
Therapeutics
disease course
bacterial communities

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Effects of fecal microbial transplantation on microbiome and immunity in simian immunodeficiency virus-infected macaques. / Hensley-McBain, Tiffany; Zevin, Alexander S.; Manuzak, Jennifer; Smith, Elise; Gile, Jillian; Miller, Charlene; Agricola, Brian; Katze, Michael; Reeves, Keith; Kraft, Colleen S.; Langevin, Stanley; Klatta, Nichole R.

In: Journal of Virology, Vol. 90, No. 10, 01.05.2016, p. 4981-4989.

Research output: Contribution to journalArticle

Hensley-McBain, T, Zevin, AS, Manuzak, J, Smith, E, Gile, J, Miller, C, Agricola, B, Katze, M, Reeves, K, Kraft, CS, Langevin, S & Klatta, NR 2016, 'Effects of fecal microbial transplantation on microbiome and immunity in simian immunodeficiency virus-infected macaques', Journal of Virology, vol. 90, no. 10, pp. 4981-4989. https://doi.org/10.1128/JVI.00099-16
Hensley-McBain, Tiffany ; Zevin, Alexander S. ; Manuzak, Jennifer ; Smith, Elise ; Gile, Jillian ; Miller, Charlene ; Agricola, Brian ; Katze, Michael ; Reeves, Keith ; Kraft, Colleen S. ; Langevin, Stanley ; Klatta, Nichole R. / Effects of fecal microbial transplantation on microbiome and immunity in simian immunodeficiency virus-infected macaques. In: Journal of Virology. 2016 ; Vol. 90, No. 10. pp. 4981-4989.
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