TY - JOUR
T1 - Effects of extracts from Italian medicinal plants on planktonic growth, biofilm formation and adherence of methicillin-resistant Staphylococcus aureus
AU - Quave, Cassandra L.
AU - Plano, Lisa R.W.
AU - Pantuso, Traci
AU - Bennett, Bradley C.
N1 - Funding Information:
The project described was funded supported by Grant Number 1F31AT004288-01A1 from the National Center for Complementary and Alternative Medicine. The content is solely the responsibility of the authors and does not necessarily reflect the official views of the National Center for Complementary and Alternative Medicine or the National Institutes of Health. Additional support for this project was provided by MBRS RISE - NIH/NIGMS R25GM061347, Botany in Action, Anne Chatham Fellowship in Medicinal Botany, USDA CSREES 20053842215940 and NIH/NCAAM 1T32AT01060-01.
PY - 2008/8/13
Y1 - 2008/8/13
N2 - Aim of study: One-third of botanical remedies from southern Italy are used to treat skin and soft tissue infection (SSTI). Staphylococcus aureus, a common cause of SSTI, has generated increasing concern due to drug resistance. Many plants possess antimicrobial agents and provide effective remedies for SSTI. Our aim was to investigate plants from different ethnobotanical usage groups for inhibition of growth and biofilms in methicillin-resistant Staphylococcus aureus (MRSA). Materials and methods: Three groups were assessed: plant remedies for SSTI, plant remedies not involving the skin, and plants with no ethnomedical application. We screened 168 extracts, representing 104 botanical species, for activity against MRSA (ATCC 33593). We employed broth dilution methods to determine the MIC after 18 h growth using an optical density (OD600nm) reading. Anti-biofilm effects were assessed by growing biofilms for 40 h, then fixing and staining with crystal violet. After washing, 10% Tween 80 was added and OD570nm readings were taken. Results: Extracts from 10 plants exhibited an IC50 ≤ 32 μg/ml for biofilm inhibition: Lonicera alpigena, Castanea sativa, Juglans regia, Ballota nigra, Rosmarinus officinalis, Leopoldia comosa, Malva sylvestris, Cyclamen hederifolium, Rosa canina, and Rubus ulmifolius. Limited bacteriostatic activity was evident. Conclusion: This study has demonstrated that the anti-biofilm activity of medicinal plants used for SSTI is significantly greater than plants without any ethnomedical applications.
AB - Aim of study: One-third of botanical remedies from southern Italy are used to treat skin and soft tissue infection (SSTI). Staphylococcus aureus, a common cause of SSTI, has generated increasing concern due to drug resistance. Many plants possess antimicrobial agents and provide effective remedies for SSTI. Our aim was to investigate plants from different ethnobotanical usage groups for inhibition of growth and biofilms in methicillin-resistant Staphylococcus aureus (MRSA). Materials and methods: Three groups were assessed: plant remedies for SSTI, plant remedies not involving the skin, and plants with no ethnomedical application. We screened 168 extracts, representing 104 botanical species, for activity against MRSA (ATCC 33593). We employed broth dilution methods to determine the MIC after 18 h growth using an optical density (OD600nm) reading. Anti-biofilm effects were assessed by growing biofilms for 40 h, then fixing and staining with crystal violet. After washing, 10% Tween 80 was added and OD570nm readings were taken. Results: Extracts from 10 plants exhibited an IC50 ≤ 32 μg/ml for biofilm inhibition: Lonicera alpigena, Castanea sativa, Juglans regia, Ballota nigra, Rosmarinus officinalis, Leopoldia comosa, Malva sylvestris, Cyclamen hederifolium, Rosa canina, and Rubus ulmifolius. Limited bacteriostatic activity was evident. Conclusion: This study has demonstrated that the anti-biofilm activity of medicinal plants used for SSTI is significantly greater than plants without any ethnomedical applications.
KW - Biofilms
KW - Italy
KW - Medicinal plants
KW - Methicillin-resistant Staphylococcus aureus
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U2 - 10.1016/j.jep.2008.05.005
DO - 10.1016/j.jep.2008.05.005
M3 - Article
C2 - 18556162
AN - SCOPUS:47749094971
VL - 118
SP - 418
EP - 428
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
SN - 0378-8741
IS - 3
ER -