Abstract
The objective of this study was to examine the effects of cyclic compressive loading on chondrogenic differentiation of rabbit bone-marrow mesenchymal stem cells (BM-MSCs) in agarose cultures. Rabbit BM-MSCs were obtained from the tibias and femurs of New Zealand white rabbits. After the chondrogenic potential of BM-MSCs was verified by pellet cultures, cell-agarose constructs were made by suspending BM-MSCs in 2% agarose (107 cells/ml) for a cyclic, unconfined compression test performed in a custom-made bioreactor. Specimens were divided into four groups: control; transforming growth factor (TGF-β) (with TGF-β1 treatment); loading (with stimulation of cyclic, unconfined compressive loading); and TGF-β loading (with TGF-β1 treatment and loading stimulation) groups. In the loading experiment, specimens were subjected to sinusoidal loading with a 10% strain magnitude at a frequency of 1 Hz for 4 hours a day. Experiments were conducted for 3, 7, and 14 consecutive days. While the experimental groups (TGF-β, loading, and TGF-β loading) exhibited significantly higher levels of expressions of chondrogenic markers (collagen II and aggrecan) at three time periods, there were no differences among the experimental groups after an extra 5-day culture. This suggests that compressive loading alone induces chondrogenic differentiation of rabbit BM-MSCs as effectively as TGF-β or TGF-β plus loading treatment. Moreover, both the compressive loading and the TGF-β1 treatment were found to promote the TGF-β1 gene expression of rabbit BM-MSCs. These findings suggest that cyclic compressive loading can promote the chondrogenesis of rabbit BM-MSCs by inducing the synthesis of TGF-β1, which can stimulate the BM-MSCs to differentiate into chondrocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 313-323 |
| Number of pages | 11 |
| Journal | Stem Cells |
| Volume | 22 |
| Issue number | 3 |
| State | Published - Jun 18 2004 |
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Keywords
- Bone marrow
- Chondrogenesis
- Compressive loading
- Mesenchymal stem cells
- TGF-β1
ASJC Scopus subject areas
- Cell Biology
Cite this
Effects of cyclic compressive loading on chondrogenesis of rabbit bone-marrow derived mesenchymal stem cells. / Huang, Chun-Yuh; Hagar, Kristen L.; Frost, Lauren E.; Sun, Yubo; Cheung, Herman S.
In: Stem Cells, Vol. 22, No. 3, 18.06.2004, p. 313-323.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of cyclic compressive loading on chondrogenesis of rabbit bone-marrow derived mesenchymal stem cells
AU - Huang, Chun-Yuh
AU - Hagar, Kristen L.
AU - Frost, Lauren E.
AU - Sun, Yubo
AU - Cheung, Herman S
PY - 2004/6/18
Y1 - 2004/6/18
N2 - The objective of this study was to examine the effects of cyclic compressive loading on chondrogenic differentiation of rabbit bone-marrow mesenchymal stem cells (BM-MSCs) in agarose cultures. Rabbit BM-MSCs were obtained from the tibias and femurs of New Zealand white rabbits. After the chondrogenic potential of BM-MSCs was verified by pellet cultures, cell-agarose constructs were made by suspending BM-MSCs in 2% agarose (107 cells/ml) for a cyclic, unconfined compression test performed in a custom-made bioreactor. Specimens were divided into four groups: control; transforming growth factor (TGF-β) (with TGF-β1 treatment); loading (with stimulation of cyclic, unconfined compressive loading); and TGF-β loading (with TGF-β1 treatment and loading stimulation) groups. In the loading experiment, specimens were subjected to sinusoidal loading with a 10% strain magnitude at a frequency of 1 Hz for 4 hours a day. Experiments were conducted for 3, 7, and 14 consecutive days. While the experimental groups (TGF-β, loading, and TGF-β loading) exhibited significantly higher levels of expressions of chondrogenic markers (collagen II and aggrecan) at three time periods, there were no differences among the experimental groups after an extra 5-day culture. This suggests that compressive loading alone induces chondrogenic differentiation of rabbit BM-MSCs as effectively as TGF-β or TGF-β plus loading treatment. Moreover, both the compressive loading and the TGF-β1 treatment were found to promote the TGF-β1 gene expression of rabbit BM-MSCs. These findings suggest that cyclic compressive loading can promote the chondrogenesis of rabbit BM-MSCs by inducing the synthesis of TGF-β1, which can stimulate the BM-MSCs to differentiate into chondrocytes.
AB - The objective of this study was to examine the effects of cyclic compressive loading on chondrogenic differentiation of rabbit bone-marrow mesenchymal stem cells (BM-MSCs) in agarose cultures. Rabbit BM-MSCs were obtained from the tibias and femurs of New Zealand white rabbits. After the chondrogenic potential of BM-MSCs was verified by pellet cultures, cell-agarose constructs were made by suspending BM-MSCs in 2% agarose (107 cells/ml) for a cyclic, unconfined compression test performed in a custom-made bioreactor. Specimens were divided into four groups: control; transforming growth factor (TGF-β) (with TGF-β1 treatment); loading (with stimulation of cyclic, unconfined compressive loading); and TGF-β loading (with TGF-β1 treatment and loading stimulation) groups. In the loading experiment, specimens were subjected to sinusoidal loading with a 10% strain magnitude at a frequency of 1 Hz for 4 hours a day. Experiments were conducted for 3, 7, and 14 consecutive days. While the experimental groups (TGF-β, loading, and TGF-β loading) exhibited significantly higher levels of expressions of chondrogenic markers (collagen II and aggrecan) at three time periods, there were no differences among the experimental groups after an extra 5-day culture. This suggests that compressive loading alone induces chondrogenic differentiation of rabbit BM-MSCs as effectively as TGF-β or TGF-β plus loading treatment. Moreover, both the compressive loading and the TGF-β1 treatment were found to promote the TGF-β1 gene expression of rabbit BM-MSCs. These findings suggest that cyclic compressive loading can promote the chondrogenesis of rabbit BM-MSCs by inducing the synthesis of TGF-β1, which can stimulate the BM-MSCs to differentiate into chondrocytes.
KW - Bone marrow
KW - Chondrogenesis
KW - Compressive loading
KW - Mesenchymal stem cells
KW - TGF-β1
UR - http://www.scopus.com/inward/record.url?scp=2942553906&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2942553906&partnerID=8YFLogxK
M3 - Article
C2 - 15153608
AN - SCOPUS:2942553906
VL - 22
SP - 313
EP - 323
JO - Stem Cells
JF - Stem Cells
SN - 1066-5099
IS - 3
ER -