Effects of cyclic compressive loading on chondrogenesis of rabbit bone-marrow derived mesenchymal stem cells

Chun-Yuh Huang, Kristen L. Hagar, Lauren E. Frost, Yubo Sun, Herman S Cheung

Research output: Contribution to journalArticle

301 Citations (Scopus)

Abstract

The objective of this study was to examine the effects of cyclic compressive loading on chondrogenic differentiation of rabbit bone-marrow mesenchymal stem cells (BM-MSCs) in agarose cultures. Rabbit BM-MSCs were obtained from the tibias and femurs of New Zealand white rabbits. After the chondrogenic potential of BM-MSCs was verified by pellet cultures, cell-agarose constructs were made by suspending BM-MSCs in 2% agarose (107 cells/ml) for a cyclic, unconfined compression test performed in a custom-made bioreactor. Specimens were divided into four groups: control; transforming growth factor (TGF-β) (with TGF-β1 treatment); loading (with stimulation of cyclic, unconfined compressive loading); and TGF-β loading (with TGF-β1 treatment and loading stimulation) groups. In the loading experiment, specimens were subjected to sinusoidal loading with a 10% strain magnitude at a frequency of 1 Hz for 4 hours a day. Experiments were conducted for 3, 7, and 14 consecutive days. While the experimental groups (TGF-β, loading, and TGF-β loading) exhibited significantly higher levels of expressions of chondrogenic markers (collagen II and aggrecan) at three time periods, there were no differences among the experimental groups after an extra 5-day culture. This suggests that compressive loading alone induces chondrogenic differentiation of rabbit BM-MSCs as effectively as TGF-β or TGF-β plus loading treatment. Moreover, both the compressive loading and the TGF-β1 treatment were found to promote the TGF-β1 gene expression of rabbit BM-MSCs. These findings suggest that cyclic compressive loading can promote the chondrogenesis of rabbit BM-MSCs by inducing the synthesis of TGF-β1, which can stimulate the BM-MSCs to differentiate into chondrocytes.

Original languageEnglish
Pages (from-to)313-323
Number of pages11
JournalStem Cells
Volume22
Issue number3
StatePublished - Jun 18 2004

Fingerprint

Chondrogenesis
Transforming Growth Factors
Mesenchymal Stromal Cells
Bone Marrow
Rabbits
Sepharose
Aggrecans
Bioreactors
Chondrocytes
Tibia
Femur
Collagen
Cell Culture Techniques
Gene Expression

Keywords

  • Bone marrow
  • Chondrogenesis
  • Compressive loading
  • Mesenchymal stem cells
  • TGF-β1

ASJC Scopus subject areas

  • Cell Biology

Cite this

Effects of cyclic compressive loading on chondrogenesis of rabbit bone-marrow derived mesenchymal stem cells. / Huang, Chun-Yuh; Hagar, Kristen L.; Frost, Lauren E.; Sun, Yubo; Cheung, Herman S.

In: Stem Cells, Vol. 22, No. 3, 18.06.2004, p. 313-323.

Research output: Contribution to journalArticle

@article{46e91ea45ceb4e6d8820d06aa658c974,
title = "Effects of cyclic compressive loading on chondrogenesis of rabbit bone-marrow derived mesenchymal stem cells",
abstract = "The objective of this study was to examine the effects of cyclic compressive loading on chondrogenic differentiation of rabbit bone-marrow mesenchymal stem cells (BM-MSCs) in agarose cultures. Rabbit BM-MSCs were obtained from the tibias and femurs of New Zealand white rabbits. After the chondrogenic potential of BM-MSCs was verified by pellet cultures, cell-agarose constructs were made by suspending BM-MSCs in 2{\%} agarose (107 cells/ml) for a cyclic, unconfined compression test performed in a custom-made bioreactor. Specimens were divided into four groups: control; transforming growth factor (TGF-β) (with TGF-β1 treatment); loading (with stimulation of cyclic, unconfined compressive loading); and TGF-β loading (with TGF-β1 treatment and loading stimulation) groups. In the loading experiment, specimens were subjected to sinusoidal loading with a 10{\%} strain magnitude at a frequency of 1 Hz for 4 hours a day. Experiments were conducted for 3, 7, and 14 consecutive days. While the experimental groups (TGF-β, loading, and TGF-β loading) exhibited significantly higher levels of expressions of chondrogenic markers (collagen II and aggrecan) at three time periods, there were no differences among the experimental groups after an extra 5-day culture. This suggests that compressive loading alone induces chondrogenic differentiation of rabbit BM-MSCs as effectively as TGF-β or TGF-β plus loading treatment. Moreover, both the compressive loading and the TGF-β1 treatment were found to promote the TGF-β1 gene expression of rabbit BM-MSCs. These findings suggest that cyclic compressive loading can promote the chondrogenesis of rabbit BM-MSCs by inducing the synthesis of TGF-β1, which can stimulate the BM-MSCs to differentiate into chondrocytes.",
keywords = "Bone marrow, Chondrogenesis, Compressive loading, Mesenchymal stem cells, TGF-β1",
author = "Chun-Yuh Huang and Hagar, {Kristen L.} and Frost, {Lauren E.} and Yubo Sun and Cheung, {Herman S}",
year = "2004",
month = "6",
day = "18",
language = "English",
volume = "22",
pages = "313--323",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "AlphaMed Press",
number = "3",

}

TY - JOUR

T1 - Effects of cyclic compressive loading on chondrogenesis of rabbit bone-marrow derived mesenchymal stem cells

AU - Huang, Chun-Yuh

AU - Hagar, Kristen L.

AU - Frost, Lauren E.

AU - Sun, Yubo

AU - Cheung, Herman S

PY - 2004/6/18

Y1 - 2004/6/18

N2 - The objective of this study was to examine the effects of cyclic compressive loading on chondrogenic differentiation of rabbit bone-marrow mesenchymal stem cells (BM-MSCs) in agarose cultures. Rabbit BM-MSCs were obtained from the tibias and femurs of New Zealand white rabbits. After the chondrogenic potential of BM-MSCs was verified by pellet cultures, cell-agarose constructs were made by suspending BM-MSCs in 2% agarose (107 cells/ml) for a cyclic, unconfined compression test performed in a custom-made bioreactor. Specimens were divided into four groups: control; transforming growth factor (TGF-β) (with TGF-β1 treatment); loading (with stimulation of cyclic, unconfined compressive loading); and TGF-β loading (with TGF-β1 treatment and loading stimulation) groups. In the loading experiment, specimens were subjected to sinusoidal loading with a 10% strain magnitude at a frequency of 1 Hz for 4 hours a day. Experiments were conducted for 3, 7, and 14 consecutive days. While the experimental groups (TGF-β, loading, and TGF-β loading) exhibited significantly higher levels of expressions of chondrogenic markers (collagen II and aggrecan) at three time periods, there were no differences among the experimental groups after an extra 5-day culture. This suggests that compressive loading alone induces chondrogenic differentiation of rabbit BM-MSCs as effectively as TGF-β or TGF-β plus loading treatment. Moreover, both the compressive loading and the TGF-β1 treatment were found to promote the TGF-β1 gene expression of rabbit BM-MSCs. These findings suggest that cyclic compressive loading can promote the chondrogenesis of rabbit BM-MSCs by inducing the synthesis of TGF-β1, which can stimulate the BM-MSCs to differentiate into chondrocytes.

AB - The objective of this study was to examine the effects of cyclic compressive loading on chondrogenic differentiation of rabbit bone-marrow mesenchymal stem cells (BM-MSCs) in agarose cultures. Rabbit BM-MSCs were obtained from the tibias and femurs of New Zealand white rabbits. After the chondrogenic potential of BM-MSCs was verified by pellet cultures, cell-agarose constructs were made by suspending BM-MSCs in 2% agarose (107 cells/ml) for a cyclic, unconfined compression test performed in a custom-made bioreactor. Specimens were divided into four groups: control; transforming growth factor (TGF-β) (with TGF-β1 treatment); loading (with stimulation of cyclic, unconfined compressive loading); and TGF-β loading (with TGF-β1 treatment and loading stimulation) groups. In the loading experiment, specimens were subjected to sinusoidal loading with a 10% strain magnitude at a frequency of 1 Hz for 4 hours a day. Experiments were conducted for 3, 7, and 14 consecutive days. While the experimental groups (TGF-β, loading, and TGF-β loading) exhibited significantly higher levels of expressions of chondrogenic markers (collagen II and aggrecan) at three time periods, there were no differences among the experimental groups after an extra 5-day culture. This suggests that compressive loading alone induces chondrogenic differentiation of rabbit BM-MSCs as effectively as TGF-β or TGF-β plus loading treatment. Moreover, both the compressive loading and the TGF-β1 treatment were found to promote the TGF-β1 gene expression of rabbit BM-MSCs. These findings suggest that cyclic compressive loading can promote the chondrogenesis of rabbit BM-MSCs by inducing the synthesis of TGF-β1, which can stimulate the BM-MSCs to differentiate into chondrocytes.

KW - Bone marrow

KW - Chondrogenesis

KW - Compressive loading

KW - Mesenchymal stem cells

KW - TGF-β1

UR - http://www.scopus.com/inward/record.url?scp=2942553906&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942553906&partnerID=8YFLogxK

M3 - Article

C2 - 15153608

AN - SCOPUS:2942553906

VL - 22

SP - 313

EP - 323

JO - Stem Cells

JF - Stem Cells

SN - 1066-5099

IS - 3

ER -