Effects of copper, cadmium, and zinc on the hatching success of brine shrimp (Artemia franciscana)

K. V. Brix, R. M. Gerdes, W. J. Adams, Martin Grosell

Research output: Contribution to journalArticle

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Abstract

Previous studies indicate that the hatching success of brine shrimp (Artemia franciscana) cysts is surprisingly sensitive to ambient metal concentrations. These studies estimated median effective concentrations (EC 50s) of 7, 5, and 28 μg l-1 for Cd, Cu, and Zn, suggesting that the hatching end point for A. franciscana is the most sensitive tested to date for Cd and Zn in saline environments and comparable in sensitivity with the most sensitive tested to date for Cu. Furthermore, these data suggest that brine shrimp are at significant risk from Cu and Zn in Great Salt Lake (GSL), UT, where ambient concentrations as high as 10 and 14 μg l-1, respectively, have been measured. Given that brine shrimp appear to be successfully reproducing in GSL, we hypothesized that these toxicity values were either biased low as a result of an artifact of the test method used or that site-specific water-quality conditions in the lake had decreased metal bioavailability such that brine shrimp could successfully reproduce. To test these hypotheses, we initiated a step-wise series of experiments. First we investigated the effects of pretreatment of brine shrimp cysts with antibiotics on brine shrimp sensitivity to metals because previous investigators as part of their test methods have used antibiotics. Next we considered the effect of ionic composition of the artificial test media on sensitivity. Finally, we evaluated the effects of the site-specific water quality of the GSL on metal bioavailability and toxicity. Results indicate that pretreatment of cysts with antibiotics had no effect on sensitivity. However, we were unable to repeat the previous values for Cd and Zn, obtaining EC50s of 11,859 and 289 μg l-1 for Cd and Zn, respectively. For Cu, however, we estimated an EC50 of 12 μg l-1, so we conducted further testing on the artificial media, adjusting the media composition to better reflect the Ca2+ and HCO 3 - concentration of normal seawater. This increased the EC50 to 28 μg l-1. Finally we evaluated the toxicity of Cu in GSL water and obtained an EC50 of 68 μg l-1, suggesting that the increased dissolved organic carbon in GSL has a significant protective effect. Overall, the results of this study suggest that brine shrimp hatching success is not particularly sensitive to Cd and Zn, but it is sensitive to Cu. However, site-specific water-quality conditions ensure that brine shrimp cyst hatching success is not significantly affected by any of these metals at the normal background concentrations that occur in GSL (<15 μg l-1).

Original languageEnglish
Pages (from-to)580-583
Number of pages4
JournalArchives of Environmental Contamination and Toxicology
Volume51
Issue number4
DOIs
StatePublished - Nov 1 2006

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Dolphins
Artemia
Cadmium
brine
Lakes
hatching
Zinc
Copper
saline lake
cadmium
zinc
copper
Salts
cyst
Metals
Antibiotics
Water quality
Cysts
Toxicity
Water Quality

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Toxicology

Cite this

Effects of copper, cadmium, and zinc on the hatching success of brine shrimp (Artemia franciscana). / Brix, K. V.; Gerdes, R. M.; Adams, W. J.; Grosell, Martin.

In: Archives of Environmental Contamination and Toxicology, Vol. 51, No. 4, 01.11.2006, p. 580-583.

Research output: Contribution to journalArticle

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