Effects of cholinergic and adrenergic agonists on adenylate cyclase activity of retinal microvascular pericytes in culture

G. Ferrari-Dileo, E. B. Davis, D. R. Anderson

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Pericytes are contractile cells that might help regulate microvascular blood flow. To understand their potential role in the regulatory responses of the retina and optic nerve head vessels, the response of pericytes isolated from bovine retinal microvessels was determined to oxotremorine, isoproterenol, phenylephrine, and clonidine. Isoproterenol doubled the basal levels of cyclic adenosine monophosphate (cAMP) specifically through β- adrenergic receptors, because the effect was blocked by dl-propranolol. The α1 agonist phenylephrine did not induce any major change in adenylate cyclase activity. The α2 agonist clonidine decreased basal cAMP synthesis and reduced the effect of isoproterenol. The cholinergic agonist oxotremorine did not modify the basal activity of adenylate cyclase but was able to decrease by almost 50% the forskolin-induced increase of cAMP. These results suggest that pericytes have functional adrenergic and cholinergic receptors, and they might respond to autonomic vasoactive substances present in vivo.

Original languageEnglish (US)
Pages (from-to)42-47
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume33
Issue number1
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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