Effects of changing operators and instruments on time-domain and spectral-domain OCT measurements of retinal nerve fiber layer thickness

Jean Claude Mwanza, Mohamed G. Gendy, William J Feuer, Wei Shi, Donald L. Budenz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

■ BACKGROUND AND OBJECTIVE: To determine the amount of interoperator and interinstrument variability introduced into retinal nerve fiber layer (RNFL) thickness measured with Stratus OCT and Cirrus HD-OCT (Carl Zeiss Meditec, Dublin, CA) in normal and glaucomatous eyes. ■ PATIENTS AND METHODS: Thirty-seven eyes from 37 subjects (20 normal and 17 with glaucoma) were included in the study. Each eye was scanned with one Stratus OCT and one Cirrus OCT by two operators. The eyes were then scanned with a different Stratus OCT and Cirrus OCT by one of the operators. Average, quadrant, and clock-hour RNFL measurements were compared between operators and between instruments after determining the proportion of variance due to subjects, operators, and instruments by means of variance component analysis. ■ RESULTS: Significant differences were observed between Stratus OCT instruments, but not betweenCirrus OCT instruments, in average (P = .01), inferior (P = .006), and nasal (P <.001) quadrant RNFL thickness in glaucomatous eyes. Interoperator differences in RNFL thickness measured with both types of instruments were non-significant. The interoperator variability ranged from 0% to 1% on Stratus OCT and was 0% on Cirrus OCT, whereas the interinstrument variability ranged from 0% to 3% for Stratus OCT and from 0% to 1% for Cirrus OCT. ■ CONCLUSION: The interoperator variability of RNFL thickness measured with Stratus OCT and Cirrus OCT, as well as the interinstrument variability of measurements obtained with Cirrus OCT, are non-significant. However, there is significant variability between RNFL measurements performed on two different Stratus OCT instruments.

Original languageEnglish (US)
Pages (from-to)328-337
Number of pages10
JournalOphthalmic Surgery Lasers and Imaging
Volume42
Issue number4
DOIs
StatePublished - Jul 2011

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Nerve Fibers
Nose
Glaucoma
Analysis of Variance

ASJC Scopus subject areas

  • Ophthalmology
  • Surgery

Cite this

Effects of changing operators and instruments on time-domain and spectral-domain OCT measurements of retinal nerve fiber layer thickness. / Mwanza, Jean Claude; Gendy, Mohamed G.; Feuer, William J; Shi, Wei; Budenz, Donald L.

In: Ophthalmic Surgery Lasers and Imaging, Vol. 42, No. 4, 07.2011, p. 328-337.

Research output: Contribution to journalArticle

Mwanza, Jean Claude ; Gendy, Mohamed G. ; Feuer, William J ; Shi, Wei ; Budenz, Donald L. / Effects of changing operators and instruments on time-domain and spectral-domain OCT measurements of retinal nerve fiber layer thickness. In: Ophthalmic Surgery Lasers and Imaging. 2011 ; Vol. 42, No. 4. pp. 328-337.
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