Effects of carcinogenesis on colonic thymidine kinase activity in familial adenomatous polyposis

Shinobu Sakamoto, Noriyuki Kasahara, Hideki Kudo, Takeo Iwama

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Thymidine kinase (TK) activity of polyp tissue from patients with familial adenomatous polyposis (FAP) was measured and compared with that of non-tumorous colon, sporadic polyp and colorectal carcinoma tissues. Total TK activity in colonic carcinoma was 3-fold that of non-tumorous colon; its increase seems attributable mainly to increased activity of cytosolic TK isozyme activity. By using DEAE-cellulose column chromatography, the colorectal TK isozymes were separated into two types, i.e. two isozymes, referred to as the fetal (predominantly cytosolic) and adult (predominantly mito-chondrial) types. However, FAP polyp samples from 15 patients showed an average elevation of only 1.8-fold over non-tumorous region of colon. Examined individually, only 5 of the 15 FAP samples showed significant elevations in total TK activity. Furthermore, TK isozyme analysis revealed variable patterns of the cytosolic isozyme activity, which was elevated in some cases (8/15) and remained low in others. Thus FAP polyps seem to be a heterogeneous population with respect to DNA replicative activity, and cytosolic TK isozyme activity may constitute a biochemical marker for the subsequent development of colorectal carcinoma in FAP.

Original languageEnglish (US)
Pages (from-to)873-876
Number of pages4
JournalCarcinogenesis
Volume13
Issue number5
DOIs
StatePublished - May 1 1992
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research

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